Poly (l-lactic acid)-based modified nanofibrous membrane with dual drug release capability for GBR application.
Poly (l-lactic acid)-based modified nanofibrous membrane with dual drug release capability for GBR application.
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聚(L-乳酸)基改性纳米纤维膜具有双重药物释放能力，可用于支架修复。
DOI：
10.1016/j.ijbiomac.2023.123201
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发表时间：
2023-03-15
影响因子：
8.2
通讯作者：
Tayebi, Lobat
中科院分区：
文献类型：
--
作者：
Shakeri, Haniyeh;Nazarpak, Masoumeh Haghbin;Imani, Rana;Tayebi, Lobat
本研究开发了一种新设计的电纺多层纳米纤维引导骨再生（GBR）。合成的多层GBR由两个不同的层组成。将辛伐他汀（SIM）与聚乳酸（PLA）复合，制成PLA/SIM复合层，用于骨缺损的修复。此外，将含有百里酚（THY）的亲水性明胶（GT）制成GT/THY层，以接触结缔组织，可能用于细菌聚集。由于亲水层和疏水层的化学性质不同且结合力较弱，因此将PLA/SIM和GT/THY杂化纤维作为亲水层和疏水层之间的结合促进剂进行静电纺丝。评价了GT/PLA多层基质的微观结构和特性，并制备了不同的纤维单分子膜以确定药物的最佳浓度。扫描电子显微镜（SEM）图像显示连续，光滑，随机排列，无珠纤维。此外，在纤维表面上没有药物颗粒，这显示了那些在纤维内部的良好放置。拉伸强度为4.60 ± 0.14 MPa，孔隙率<50%，孔径大小适中。通过体外溶胀和降解性能的表征，证实了其具有良好的溶胀性能（293 ± 0.05%）和降解速率. GT/PLA膜具有较长的SIM释放时间和较长的缓释时间，并具有较高的抗菌活性。细胞活性、细胞贴壁实验和4′，6-diamidino-2-phenylindole（DAPI）核染色结果表明，所设计的GT/PLA基质具有良好的生物相容性和细胞贴壁性。细胞浸润测试还显示，细胞被外层（GT/THY）精细地阻止。总体而言，本研究中获得的结果表明，制备的GT/PLA具有用作GBR的巨大潜力，可以开发成骨和抗菌仿生骨膜，优化GBR策略的临床应用。
Electrospun multilayer nanofibers guided bone regeneration (GBR) with a new design were developed in this study. The synthesized multilayer GBR was composed of two distinct layers. Poly l-lactic acid (PLA) incorporated with simvastatin (SIM) was designed as PLA/SIM layer to contact with a bone defect. In addition, the hydrophilic gelatin (GT) containing thymol (THY) was fabricated as GT/THY layer to contact connective tissue, potentially for bacterial gathering. Due to the different chemical nature and weak cohesion of the hydrophilic and hydrophobic layers, hybrid fibers made of PLA/SIM and GT/THY were electrospun as cohesion promoters between these layers. The microstructure and characteristics of the synthesized multilayer substrate, named GT/PLA, were evaluated, and different fibrous monolayers were fabricated to determine the optimal concentrations of drugs. Scanning electron microscopy (SEM) images showed continuous, smooth, randomly aligned, and bead-free fibers. In addition, there were no drug particles on the fiber surfaces which displayed the good placement of those inside the fibers. The mats exhibited satisfactory tensile strength (4.60 ± 0.14 MPa) and favorable physicochemical properties, including proper porosity percentage (<50 %) and appropriate pore size. Suitable swelling behavior (293 ± 0.05 %) and adequate degradation rates were also approved by characterizing swelling and degradability in vitro. The GT/PLA membrane exhibited a prolonged and sustained SIM release and controlled THY release with high antibacterial efficiency. Cell viability, cell attachment assay, and nuclear staining using 4′,6-diamidino-2-phenylindole (DAPI) showed that the designed GT/PLA substrate had good biocompatibility and cell attachment. Cell infiltration testing also showed that the cells were finely prevented by the outer layer (GT/THY). Overall, the obtained results in this study indicated the great potential of the prepared GT/PLA for use as a GBR which can develop osteogenic and antibacterial biomimetic periosteum optimizing the clinical application of GBR strategies.
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具有屏障功能和促进成骨作用的双层聚乳酸-乙醇酸/纳米羟基磷灰石膜，用于引导骨再生
DOI：
10.3390/ma10030257
发表时间：
2017-03-03
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Fu L;Wang Z;Dong S;Cai Y;Ni Y;Zhang T;Wang L;Zhou Y
通讯作者：
Zhou Y
影响因子：
9.7
作者：
Chong, E. J.;Phan, T. T.;Lim, C. T.
通讯作者：
Lim, C. T.
DOI：
10.1111/j.1749-6632.2002.tb03041.x
发表时间：
2002-01-01
期刊：
REPARATIVE MEDICINE: GROWING TISSUES AND ORGANS
影响因子：
--
作者：
Godbey, WT;Atala, A
通讯作者：
Atala, A
影响因子：
14
作者：
Ji, Wei;Yang, Fang;Jansen, John A.
通讯作者：
Jansen, John A.
影响因子：
5.8
作者：
Charernsriwilaiwat, Natthan;Rojanarata, Theerasak;Opanasopit, Praneet
通讯作者：
Opanasopit, Praneet