Inflammatory Stress Induced by Intraperitoneal Injection of LPS Increases Phoenixin Expression and Activity in Distinct Rat Brain Nuclei.
Inflammatory Stress Induced by Intraperitoneal Injection of LPS Increases Phoenixin Expression and Activity in Distinct Rat Brain Nuclei.
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腹膜内注射LPS引起的炎症应激会增加凤凰蛋白的表达和不同大鼠脑核的活性。
DOI：
10.3390/brainsci12020135
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发表时间：
2022-01-20
期刊：
影响因子：
3.3
通讯作者：
Stengel A
中科院分区：
文献类型：
--
作者：
Friedrich T;Schalla MA;Goebel-Stengel M;Kobelt P;Rose M;Stengel A
由于菲尼克斯蛋白在束缚应激和糖皮质激素应激中的作用，以及最近显示的对炎症小体的影响，我们旨在研究脂多糖（LPS）诱导的炎症应激对表达菲尼克斯蛋白的脑核活性的影响。雄性 Sprague Dawley 大鼠（n = 6/组）腹膜内注射 LPS 或对照（盐水）。对大脑进行 c-Fos 和 phoenixin 免疫组织化学处理，并使用 ImageJ 软件对所得玻片进行评估。使用光密度测定法对 c-Fos 进行计数并评估 phoenixin。 LPS应激显着增加了中央杏仁核（CeM，7.2倍）、视上核（SON，34.8±17.3 vs. 0.0±0.0）、弓状核（Arc，4.9倍）、中缝苍白核（RPa，5.1倍）、终纹床核（BSt，与对照注射组相比，迷走神经背运动核（DMN，89 倍）和孤束核内侧部分（mNTS，121 倍）（p < 0.05）。 Phoenixin 表达在 CeM（1.2 倍）、SON（1.5 倍）、RPa（1.3 倍）、DMN（1.3 倍）和 mNTS（1.9 倍，p < 0.05）中也显着增加，导致 RPa、BSt 和 mNTS 中 c-Fos 和 phoenixin 之间呈正相关（p < 0.05）。总之，脂多糖应激会导致凤凰蛋白免疫反应性脑核团的活性显着增加，这与束缚应激明显不同。
Due to phoenixin’s role in restraint stress and glucocorticoid stress, as well as its recently shown effects on the inflammasome, we aimed to investigate the effects of lipopolysaccharide (LPS)-induced inflammatory stress on the activity of brain nuclei-expressing phoenixin. Male Sprague Dawley rats (n = 6/group) were intraperitoneally injected with either LPS or control (saline). Brains were processed for c-Fos and phoenixin immunohistochemistry and the resulting slides were evaluated using ImageJ software. c-Fos was counted and phoenixin was evaluated using densitometry. LPS stress significantly increased c-Fos expression in the central amygdaloid nucleus (CeM, 7.2-fold), supraoptic nucleus (SON, 34.8 ± 17.3 vs. 0.0 ± 0.0), arcuate nucleus (Arc, 4.9-fold), raphe pallidus (RPa, 5.1-fold), bed nucleus of the stria terminalis (BSt, 5.9-fold), dorsal motor nucleus of the vagus nerve (DMN, 89-fold), and medial part of the nucleus of the solitary tract (mNTS, 121-fold) compared to the control-injected group (p < 0.05). Phoenixin expression also significantly increased in the CeM (1.2-fold), SON (1.5-fold), RPa (1.3-fold), DMN (1.3-fold), and mNTS (1.9-fold, p < 0.05), leading to a positive correlation between c-Fos and phoenixin in the RPa, BSt, and mNTS (p < 0.05). In conclusion, LPS stress induces a significant increase in activity in phoenixin immunoreactive brain nuclei that is distinctively different from restraint stress.
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DOI：
10.1152/ajpregu.00426.2017
发表时间：
2018-04-01
影响因子：
2.8
作者：
Gasparini, Silvia;Stein, Lauren M.;Samson, Willis K.
通讯作者：
Samson, Willis K.
影响因子：
9.3
作者：
Bonnet, Marion S.;Pecchi, Emilie;Troadec, Jean-Denis
通讯作者：
Troadec, Jean-Denis
影响因子：
2.9
作者：
BONAZ, B;TACHE, Y
通讯作者：
TACHE, Y
影响因子：
3.3
作者：
Huang, Jian;Wang, Yaoli;Huang, Xiankai
通讯作者：
Huang, Xiankai
影响因子：
5.3
作者：
Barros VN;Mundim M;Galindo LT;Bittencourt S;Porcionatto M;Mello LE
通讯作者：
Mello LE