Four-dimensional live imaging of hemodynamics in mammalian embryonic heart with Doppler optical coherence tomography.
Four-dimensional live imaging of hemodynamics in mammalian embryonic heart with Doppler optical coherence tomography.
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DOI：
10.1002/jbio.201500314
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发表时间：
2016-08
影响因子：
2.8
通讯作者：
Larina, Irina V.
中科院分区：
文献类型：
--
作者：
Wang, Shang;Lakomy, David S.;Garcia, Monica D.;Lopez, Andrew L., III;Larin, Kirill V.;Larina, Irina V.
关键词：
小鼠胚胎心脏的血流动力学分析对于了解早期心脏发生的功能方面和推进先天性心脏缺陷的研究是必不可少的。然而，在哺乳动物模型中心脏血流动力学的高分辨率成像仍然具有挑战性，主要是由于胚胎心脏的动态性质和深部位置。在这里，我们报告四维微尺度成像的血流在早期小鼠胚胎心脏，使时间分辨测量和分析整个心管的流速。我们的方法使用多普勒光学相干断层扫描在活的小鼠胚胎培养，并采用后处理同步的方法来重建三维数据随着时间的推移，在100 Hz的体积速率。实验在胚胎第9.0天的活小鼠胚胎上进行。我们的研究结果显示了跳动的心脏内的血流动力学，在原始心房，房室和球室区域，和球心的定量流速评估的能力。结合心脏动力学和血流动力学分析表明，这种功能成像方法可以用来进一步研究血流动力学和心脏壁运动之间的力学关系，为研究哺乳动物心脏发生早期的生物力学带来新的可能性。使用多普勒光学相干断层扫描对胚胎9.0天的小鼠胚胎心脏进行四维实时血流动力学成像，显示静脉窦、原始心房、房室区和卵黄静脉中的定向血流。先天性心脏病是最常见的先天性疾病，在美国影响1%的新生儿。早期发育的哺乳动物心脏的血流动力学的可视化和测量可以为提高对正常心脏发生和先天性心脏缺陷的理解提供很大的见解。在这项研究中，多普勒光学相干断层扫描进行活小鼠胚胎培养，以获得第一个四维高分辨率成像和测量的哺乳动物胚胎心脏的血流动力学特征。
Hemodynamic analysis of the mouse embryonic heart is essential for understanding the functional aspects of early cardiogenesis and advancing the research in congenital heart defects. However, high-resolution imaging of cardiac hemodynamics in mammalian models remains challenging, primarily due to the dynamic nature and deep location of the embryonic heart. Here we report four-dimensional micro-scale imaging of blood flow in the early mouse embryonic heart, enabling time-resolved measurement and analysis of flow velocity throughout the heart tube. Our method uses Doppler optical coherence tomography in live mouse embryo culture, and employs a post-processing synchronization approach to reconstruct three-dimensional data over time at a 100 Hz volume rate. Experiments were performed on live mouse embryos at embryonic day 9.0. Our results show blood flow dynamics inside the beating heart, with the capability for quantitative flow velocity assessment in the primitive atrium, atrioventricular and bulboventricular regions, and bulbus cordis. Combined cardiodynamic and hemodynamic analysis indicates this functional imaging method can be utilized to further investigate the mechanical relationship between blood flow dynamics and cardiac wall movement, bringing new possibilities to study biomechanics in early mammalian cardiogenesis. Four-dimensional live hemodynamic imaging of the mouse embryonic heart at embryonic day 9.0 using Doppler optical coherence tomography, showing directional blood flows in the sinus venosus, primitive atrium, atrioventricular region and vitelline vein. Congenital heart disease is the most common congenital disorder, affecting 1% newborns in the U.S. Visualization and measurement of hemodynamics in early developing mammalian heart can provide great insights for improved understanding of normal cardiogenesis and congenital cardiac defects. In this study, Doppler optical coherence tomography is performed in live mouse embryo culture to obtain the first four-dimensional high-resolution imaging and measurement of hemodynamic features in the mammalian embryonic heart.
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影响因子：
3.8
作者：
Gargesha M;Jenkins MW;Wilson DL;Rollins AM
通讯作者：
Rollins AM
影响因子：
3.5
作者：
Jenkins, Michael W.;Peterson, Lindsy;Rollins, Andrew M.
通讯作者：
Rollins, Andrew M.
影响因子：
4
作者：
Karunamuni GH;Gu S;Ford MR;Peterson LM;Ma P;Wang YT;Rollins AM;Jenkins MW;Watanabe M
通讯作者：
Watanabe M
影响因子：
2.9
作者：
Aristizábal, O;Christopher, DA;Turnbull, DH
通讯作者：
Turnbull, DH
DOI：
10.1364/josaa.25.003134
发表时间：
2008-12-01
影响因子：
1.9
作者：
Davis, A. M.;Rothenberg, F. G.;Izatt, J. A.
通讯作者：
Izatt, J. A.