A radiation hybrid map of the rat genome containing 5,255 markers

A radiation hybrid map of the rat genome containing 5,255 markers
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包含 5,255 个标记的大鼠基因组辐射杂交图谱

DOI:
10.1038/8737
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发表时间:
1999
期刊:
影响因子:
30.8
通讯作者:
M. James
M. James
中科院分区:
生物学1区
文献类型:
--
作者:
Takeshi K. Watanabe;M. Bihoreau;L. McCarthy;S. Kiguwa;H. Hishigaki;Atsushi Tsuji;J. Browne;Y. Yamasaki;A. Mizoguchi‐Miyakita;K. Oga;T. Ono;S. Okuno;N. Kanemoto;E. Takahashi;Kazuhiro Tomita;H. Hayashi;M. Adachi;C. Webber;M. Davis;Susanne Kiel;Catherine Knights;Angela L. Smith;R. Critcher;Jonathan Miller;T. Thangarajah;P. Day;J. Hudson;Yasuo Irie;T. Takagi;Yusuke Nakamura;P. Goodfellow;G. Lathrop;A. Tanigami;M. James

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采用全基因组辐射杂交(RH)技术构建基于微卫星和基因标记的高分辨率大鼠基因组图谱。其中包括首次描述的3019个新的微卫星标记和已知遗传位置的1714个微卫星标记,可以对来自不同来源的图谱进行比较和整合。一个稳健的RH框架图包含1030个位置,排序的概率至少为1000:1,已被定义为绘制这些标记的工具,并为未来大鼠的RH绘制提供了工具。超过500个在小鼠和/或人类中被定位的基因与大鼠RH框架相关,允许构建详细的大鼠-小鼠和大鼠-人比较图,并说明RH方法用于比较制图的力量。
A whole-genome radiation hybrid (RH) panel was used to construct a high-resolution map of the rat genome based on microsatellite and gene markers. These include 3,019 new microsatellite markers described here for the first time and 1,714 microsatellite markers with known genetic locations, allowing comparison and integration of maps from different sources. A robust RH framework map containing 1,030 positions ordered with odds of at least 1,000:1 has been defined as a tool for mapping these markers, and for future RH mapping in the rat. More than 500 genes which have been mapped in mouse and/or human were localized with respect to the rat RH framework, allowing the construction of detailed rat-mouse and rat-human comparative maps and illustrating the power of the RH approach for comparative mapping.
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