Localization and function of neurosecretory protein GM, a novel small secretory protein, in the chicken hypothalamus.
Localization and function of neurosecretory protein GM, a novel small secretory protein, in the chicken hypothalamus.
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DOI：
10.1038/s41598-017-18822-9
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发表时间：
2018-01-15
影响因子：
4.6
通讯作者：
Ukena K
中科院分区：
文献类型：
--
作者：
Shikano K;Bessho Y;Kato M;Iwakoshi-Ukena E;Taniuchi S;Furumitsu M;Tachibana T;Bentley GE;Kriegsfeld LJ;Ukena K
最近，我们在鸡下丘脑漏斗部发现了一个新的编码神经分泌蛋白GL（NPGL）前体的cDNA。NPGL在生长和摄食调节中起重要作用。数据库搜索表明，NPGL基因有一个旁系同源基因：神经分泌蛋白GM（NPGM），也在鸡。我们在鸡中鉴定了编码NPGM前体的cDNA。形态学分析表明，含NPGM的细胞特异性地定位于下丘脑的乳头体内侧核（MM）和漏斗核（IN）。此外，我们发现NPGM和NPGL是共定位的，特别是在MM。NPGM mRNA的表达水平逐渐下降，在孵化后的发展过程中，相反的NPGL mRNA。此外，我们还研究了NPGM与其他已知因素之间的关系。NPGM被发现产生于MM中的组胺能神经元中。NPGM和组氨酸脱羧酶（一种组胺产生酶）在孵化后发育过程中显示出相似的表达模式。急性侧脑室注射NPGM可减少摄食量，其作用与组胺相似。据我们所知，这是首次报道NPGM在脊椎动物脑中的定位和功能。这些结果将进一步推进对能量稳态机制的理解。
Recently, we discovered a novel cDNA encoding the precursor of a small secretory protein, neurosecretory protein GL (NPGL), in the hypothalamic infundibulum of chickens. NPGL plays an important role in the regulation of growth and feeding. A database search indicated that the NPGL gene has a paralogous gene: neurosecretory protein GM (NPGM), also in chickens. We identified cDNA encoding the NPGM precursor in chickens. Morphological analysis showed that NPGM-containing cells are specifically localized in the medial mammillary nucleus (MM) and infundibular nucleus (IN) in the hypothalamus. In addition, we found that NPGM and NPGL are co-localized, especially in the MM. The expression levels of NPGM mRNA gradually decreased during post-hatch development, in contrast to those of NPGL mRNA. Moreover, we investigated the relationship between NPGM and other known factors. NPGM was found to be produced in histaminergic neurons in the MM. NPGM and histidine decarboxylase, a histamine-producing enzyme, displayed similar expression patterns during post-hatch development. Acute intracerebroventricular injection of NPGM decreased food intake, similar to the effect of histamine. To our knowledge, this is the first report of the localization and function of NPGM in the brain of vertebrates. These results will further advance the understanding mechanisms underlying energy homeostasis.
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影响因子：
3.3
作者：
Yanai, K;Son, LZ;Watanabe, T
通讯作者：
Watanabe, T
影响因子：
4.3
作者：
Tachibana T;Tsutsui K
通讯作者：
Tsutsui K
影响因子：
5.3
作者：
Takahashi, Kazumi;Lin, Jian-Sheng;Sakai, Kazuya
通讯作者：
Sakai, Kazuya
影响因子：
4.8
作者：
Ukena, Kazuyoshi;Tachibana, Tetsuya;Tsutsui, Kazuyoshi
通讯作者：
Tsutsui, Kazuyoshi
影响因子：
64.8
作者：
Qu, DQ;Ludwig, DS;MaratosFlier, E
通讯作者：
MaratosFlier, E