Mucosal expression of aquaporin 5 and epithelial barrier proteins in chronic rhinosinusitis with and without nasal polyps.
Mucosal expression of aquaporin 5 and epithelial barrier proteins in chronic rhinosinusitis with and without nasal polyps.
复制标题
DOI：
10.1016/j.amjoto.2013.11.011
复制
发表时间：
2014-05
影响因子：
2.5
通讯作者：
Leid JG
中科院分区：
文献类型：
--
作者：
Shikani AH;Sidhaye VK;Basaraba RJ;Shikani HJ;Alqudah MA;Kirk N;Cope E;Leid JG
本研究的目的是探讨慢性难治性鼻窦炎(CRS)患者鼻息肉的病理生理学特征，即以黏膜水膜通透性蛋白水通道蛋白5(AQP5)的组织学变化和差异表达为代表的上皮屏障功能改变之间的关系。前瞻性临床研究。三级鼻科转诊中心。鼻窦标本取自7例CRS鼻息肉患者(CRSwNP)、7例非鼻息肉CRS患者(CRSsNP)和5例健康对照患者。通过苏木精-伊红染色膜屏障和免疫组织化学染色AQP5表达来评价粘膜的变化，AQP5是一种影响跨上皮水通透性和组织水肿的膜通道蛋白。实时荧光定量聚合酶链式反应(RT-PCR)和免疫印迹检测AQP5的表达。其他膜蛋白的水平，包括E-钙粘附素和Septin-2，也被检测。CRSwNP患者表现出大量的组织学证据，表现为膜重塑、水肿增加和腺体增生。CRSwNP组AQP5在上皮中的表达明显低于CRSsNP组和对照组。E-钙粘蛋白和Septin-2的表达差异无统计学意义。总之，这些数据表明，与对照组相比，在CRS(主要是CRSwNP)的情况下，粘膜上皮屏障受到了损害，AQP5在维持粘膜水稳态中扮演着关键的紧密连接蛋白的角色。我们推测AQP5在粘膜水肿和息肉形成的病理生理学中可能起作用。
The purpose of this study is to characterize the association between altered epithelial barrier function, represented by changes in histology and differential expression of the mucosal water membrane permeability protein aquaporin 5 (AQP5), and the pathophysiology of chronic refractory sinusitis (CRS) in patients with and without nasal polyposis. Prospective clinical study. Tertiary rhinology referral center. Sinonasal samples were obtained from seven CRS subjects with nasal polyps (CRSwNP), seven CRS without nasal polyposis (CRSsNP), and five control healthy patients. Mucosal membrane changes were evaluated through hematoxylin and eosin staining of the membrane barrier and immunohistochemical staining of AQP5 expression, a membrane channel protein that affects trans-epithelial water permeability and tissue edema. AQP5 expression was confirmed by real-time PCR (rt-PCR) and western blot. Levels of other membrane proteins, including E-cadherin and Septin-2, were also assessed. CRSwNP patients showed substantial histologic evidence of membrane remodeling with increased edema and glandular hyperplasia. The epithelial expression of AQP5 was significantly lower in CRSwNP as compared to CRSsNP or control. There was no significant difference in the expression of E-cadherin and Septin-2. Collectively, these data suggest that the mucosal epithelial barrier is compromised in the context of CRS (predominantly in CRSwNP) when compared to control and that AQP5 acts as a key tight junction protein in the maintenance of mucosal water homeostasis. We hypothesize that AQP5 plays a possible role in the pathophysiology of mucosal edema and polyp formation.
登录
查看更多内容
DOI：
10.1016/j.otohns.2004.09.067
发表时间：
2004-12
期刊：
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery
影响因子：
--
作者：
Meltzer EO;Hamilos DL;Hadley JA;Lanza DC;Marple BF;Nicklas RA;Bachert C;Baraniuk J;Baroody FM;Benninger MS;Brook I;Chowdhury BA;Druce HM;Durham S;Ferguson B;Gwaltney JM Jr;Kaliner M;Kennedy DW;Lund V;Naclerio R;Pawankar R;Piccirillo JF;Rohane P;Simon R;Slavin RG;Togias A;Wald ER;Zinreich SJ;American Academy of Allergy, Asthma and Immunology;American Academy of Otolaryngic Allergy;American Academy of Otolaryngology-Head and Neck Surgery;American College of Allergy, Asthma and Immunology;American Rhinologic Society
通讯作者：
American Rhinologic Society
影响因子：
14.2
作者：
Tieu, David D.;Kern, Robert C.;Schleimer, Robert P.
通讯作者：
Schleimer, Robert P.
DOI：
10.2500/ajr.2008.22.3235
发表时间：
2008-11-01
期刊：
AMERICAN JOURNAL OF RHINOLOGY
影响因子：
--
作者：
Zuckerman, Jodi D.;Lee, Winston Y.;Parkos, Charles A.
通讯作者：
Parkos, Charles A.
影响因子：
14.2
作者：
Kato, Atsushi;Peters, Anju;Schleimer, Robert P.
通讯作者：
Schleimer, Robert P.
DOI：
10.1513/pats.200808-088rm
发表时间：
2009-05-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Schleimer, Robert P;Kato, Atsushi;Kern, Robert C
通讯作者：
Kern, Robert C