Implications of cultured periodontal ligament cells for the clinical and experimental setting: a review.
Implications of cultured periodontal ligament cells for the clinical and experimental setting: a review.
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DOI：
10.1016/j.archoralbio.2011.03.003
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发表时间：
2011-10
影响因子：
3
通讯作者：
Kapila, Yvonne L.
中科院分区：
文献类型：
--
作者：
Marchesan, Julie Teresa;Scanlon, Christina Springstead;Soehren, Stephen;Matsuo, Masato;Kapila, Yvonne L.
牙周膜（PDL）是牙周组织再生过程中的关键因素。PDL组织的异质性、其在成年早期的发育以及PDL组织在体内暴露于的不同条件赋予PDL独特的特征，这些特征可能在其体外培养期间是重要的。影响体内环境的几个因素影响PDL成纤维细胞在培养中的行为。本综述的目的是解决不同的因素，影响PDL成纤维细胞的行为在培养-在体内-体外转换，细胞鉴定/分离标记，原代PDL文化和细胞系，牙齿特异性因素，和供体特异性因素。基于回顾的研究，作者的建议包括使用几种识别标志物来确认细胞身份，在早期传代时使用原代培养物来保持独特的PDL异质性特征，并注意供体条件，如年龄，全身健康状况和牙齿健康状况。持续的努力将扩大我们对细胞体外和体内行为的理解，目标是协调最佳的牙周再生。这种理解将导致更个性化和可预测的牙周再生治疗的改善循证理论基础。
The periodontal ligament (PDL) is a key contributor to the process of regeneration of the periodontium. The heterogeneous nature of the PDL tissue, its development during early adulthood, and the different conditions to which the PDL tissue is exposed to in vivo impart on the PDL unique characteristics that may be of consequence during its cultivation in vitro. Several factors affecting the in vivo setting influence the behavior of PDL fibroblasts in culture. The purpose of this review is to address distinct factors that influence the behavior of PDL fibroblasts in culture — in vivo-in vitro transitions, cell identification/isolation markers, primary PDL cultures and cell lines, tooth-specific factors, and donor-specific factors. Based on the reviewed studies, the authors recommendations include the use of several identification markers to confirm cell identity, use of primary cultures at early passage to maintain unique PDL heterogeneic characteristics, and noting donor conditions such as age, systemic health status, and tooth health status. Continued efforts will expand our understanding of the in vitro and in vivo behavior of cells, with the goal of orchestrating optimal periodontal regeneration. This understanding will lead to improved evidence-based rationales for more individualized and predictable periodontal regenerative therapies.
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影响因子：
3
作者：
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通讯作者：
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影响因子：
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