Ultrarapid Inductive Rewarming of Vitrified Biomaterials with Thin Metal Forms.
Ultrarapid Inductive Rewarming of Vitrified Biomaterials with Thin Metal Forms.
复制标题
薄金属形式的玻璃化生物材料的超快速感应复温
DOI：
10.1007/s10439-018-2063-1
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发表时间：
2018-11
影响因子：
3.8
通讯作者：
Bischof J
中科院分区：
文献类型：
--
作者：
Manuchehrabadi N;Shi M;Roy P;Han Z;Qiu J;Xu F;Lu TJ;Bischof J
通过加载低温保护剂（CPA）如VS 55（8.4 M）或更低浓度的DP 6（6 M）并分别以或超过其临界冷却速率2.5和40 °C/min冷却，可以成功地玻璃化壁厚为1 mm的动脉。然而，从这种玻璃化状态成功升温可能具有挑战性。例如，通过简单的温浴浸泡的对流升温达到70 °C/min，这比VS 55的临界升温速率55 °C/min快，但仍远低于DP 6的临界升温速率（185 °C/min）。在这里，我们提出了一种新的方法，可以显着增加升温速率内的任何一个解决方案或组织通过感应加热市售金属组件放置在解决方案或接近组织与非侵入性射频场（360 kHz，20 kA/m）。溶液中直接测量的升温速率超过1000 °C/min，泡沫铜、铝箔和镍钛合金网的比吸收率（W/g）分别为100、450和1000。作为原理的证明，使用铝箔以高存活率成功地使扩散地装载有VS 55和DP 6 CPA的颈动脉变暖，而装载有DP 6的组织的标准对流失败。建模表明，这种方法可以改善高达4 mm厚的组织中的升温，其中CPA的扩散加载可能不完全。最后，这项技术不依赖于系统的大小，因此应该在对流不能的地方扩大规模。本文的在线版本（10.1007/s10439-018-2063-1）包含补充材料，可供授权用户使用。
Arteries with 1-mm thick walls can be successfully vitrified by loading cryoprotective agents (CPAs) such as VS55 (8.4 M) or less concentrated DP6 (6 M) and cooling at or beyond their critical cooling rates of 2.5 and 40 °C/min, respectively. Successful warming from this vitrified state, however, can be challenging. For example, convective warming by simple warm-bath immersion achieves 70 °C/min, which is faster than VS55’s critical warming rate of 55 °C/min, but remains far below that of DP6 (185 °C/min). Here we present a new method that can dramatically increase the warming rates within either a solution or tissue by inductively warming commercially available metal components placed within solutions or in proximity to tissues with non-invasive radiofrequency fields (360 kHz, 20 kA/m). Directly measured warming rates within solutions exceeded 1000 °C/min with specific absorption rates (W/g) of 100, 450 and 1000 for copper foam, aluminum foil, and nitinol mesh, respectively. As proof of principle, a carotid artery diffusively loaded with VS55 and DP6 CPA was successfully warmed with high viability using aluminum foil, while standard convection failed for the DP6 loaded tissue. Modeling suggests this approach can improve warming in tissues up to 4-mm thick where diffusive loading of CPA may be incomplete. Finally, this technology is not dependent on the size of the system and should therefore scale up where convection cannot. The online version of this article (10.1007/s10439-018-2063-1) contains supplementary material, which is available to authorized users.
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影响因子：
3.6
作者：
Sandby-Moller, J;Poulsen, T;Wulf, HC
通讯作者：
Wulf, HC
影响因子：
4.6
作者：
STAUFFER, PR;CETAS, TC;ROEMER, RB
通讯作者：
ROEMER, RB
影响因子：
--
作者：
Etheridge, Michael L.;Xu, Yi;Bischof, John C.
通讯作者：
Bischof, John C.
影响因子：
2.7
作者：
RUGGERA, PS;FAHY, GM
通讯作者：
FAHY, GM
影响因子：
46.9
作者：
Giwa S;Lewis JK;Alvarez L;Langer R;Roth AE;Church GM;Markmann JF;Sachs DH;Chandraker A;Wertheim JA;Rothblatt M;Boyden ES;Eidbo E;Lee WPA;Pomahac B;Brandacher G;Weinstock DM;Elliott G;Nelson D;Acker JP;Uygun K;Schmalz B;Weegman BP;Tocchio A;Fahy GM;Storey KB;Rubinsky B;Bischof J;Elliott JAW;Woodruff TK;Morris GJ;Demirci U;Brockbank KGM;Woods EJ;Ben RN;Baust JG;Gao D;Fuller B;Rabin Y;Kravitz DC;Taylor MJ;Toner M
通讯作者：
Toner M