Laser Desorption/Ionization Mass Spectrometric Imaging of Endogenous Lipids from Rat Brain Tissue Implanted with Silver Nanoparticles.
Laser Desorption/Ionization Mass Spectrometric Imaging of Endogenous Lipids from Rat Brain Tissue Implanted with Silver Nanoparticles.
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DOI：
10.1007/s13361-017-1665-4
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发表时间：
2017-08
影响因子：
3.2
通讯作者：
Woods AS
中科院分区：
文献类型：
--
作者：
Muller L;Baldwin K;Barbacci DC;Jackson SN;Roux A;Balaban CD;Brinson BE;McCully MI;Lewis EK;Schultz JA;Woods AS
植入银纳米颗粒（AgNP）基质的组织的质谱成像（MSI）在啮齿动物疾病和损伤模型中产生脂质的可再现成像。气相生产和加速的大小选择的8纳米AgNP之后，受控的离子束光栅和软着陆植入500 eV AgNP到组织中。聚焦337 nm激光解吸产生高质量的图像，大鼠脑组织中的大多数脂质类（在正离子模式：半乳糖神经酰胺，二酰基甘油，神经酰胺，磷脂酰胆碱，胆固醇酯，和胆固醇，和在负离子模式：磷脂酰乙醇酰胺，硫苷脂，磷脂酰肌醇，和鞘磷脂）。通过选择银离子而不是碱性阳离子化离子，脑组织连续切片的图像再现性在<10%的公差内实现。用纯标准品点样的脑组织成像用于证明Ag阳离子化神经酰胺和二酰基甘油离子来自完整的内源性物种。相比之下，几乎所有Ag阳离子化的脂肪酸离子是具有脂肪酸作为亚基的许多脂质类型的片段化的结果。几乎没有完整的脂肪酸离子来自组织上的纯脂肪酸标准品。
Mass spectrometry imaging (MSI) of tissue implanted with silver nanoparticulate (AgNP) matrix generates reproducible imaging of lipids in rodent models of disease and injury. Gas-phase production and acceleration of size-selected 8 nm AgNP is followed by controlled ion beam rastering and soft-landing implantation of 500 eV AgNP into tissue. Focused 337 nm laser desorption produces high quality images for most lipid classes in rat brain tissue (in positive mode: galactoceramides, diacylglycerols, ceramides, phosphatidylcholines, cholesteryl ester, and cholesterol, and in negative ion mode: phosphatidylethanolamides, sulfatides, phosphatidylinositol, and sphingomyelins). Image reproducibility in serial sections of brain tissue is achieved within <10% tolerance by selecting argentated instead of alkali cationized ions. The imaging of brain tissues spotted with pure standards was used to demonstrate that Ag cationized ceramide and diacylglycerol ions are from intact, endogenous species. In contrast, almost all Ag cationized fatty acid ions are a result of fragmentations of numerous lipid types having the fatty acid as a subunit. Almost no argentated intact fatty acid ions come from the pure fatty acid standard on tissue.
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影响因子：
2
作者：
Harvey, DJ
通讯作者：
Harvey, DJ
DOI：
10.1007/s13361-011-0122-z
发表时间：
2011-06
影响因子：
3.2
作者：
Hankin JA;Farias SE;Barkley RM;Heidenreich K;Frey LC;Hamazaki K;Kim HY;Murphy RC
通讯作者：
Murphy RC
影响因子：
3
作者：
Roux A;Muller L;Jackson SN;Post J;Baldwin K;Hoffer B;Balaban CD;Barbacci D;Schultz JA;Gouty S;Cox BM;Woods AS
通讯作者：
Woods AS
影响因子：
2.5
作者：
Hua, Lin;Chen, Jianrong;Tan, Swee Ngin
通讯作者：
Tan, Swee Ngin
影响因子：
7.4
作者：
Dutta, TK;Harayama, S
通讯作者：
Harayama, S