Functionalizing DNA nanostructures with natural cationic amino acids.
Functionalizing DNA nanostructures with natural cationic amino acids.
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用天然阳离子氨基酸功能化 DNA 纳米结构
DOI：
10.1016/j.bioactmat.2021.02.012
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发表时间：
2021-09
影响因子：
18.9
通讯作者：
Qian H
中科院分区：
文献类型：
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作者：
Wang D;Chen C;Liu Q;Zhao Q;Wu D;Yuan Y;Huang C;Sun X;Huang C;Leong DT;Wang G;Qian H
将自组装的DNA纳米结构与各种功能性客体物种复合是开启新的和令人兴奋的生物医学应用的关键。阳离子客体不仅可以诱导无镁DNA自组装成特定的结构，还可以赋予最终的复合纳米材料新的性质。在此，我们提出了一种新的策略，采用天然存在的阳离子氨基酸诱导DNA自组装成定义的纳米结构。天然L-精氨酸和L-赖氨酸可以容易地以无镁的方式诱导基于瓦片的DNA纳米管和DNA折纸片的组装。的自组装过程被证明是pH值和浓度依赖性，并在恒温下实现。而且，组装的DNA/氨基酸复合物纳米材料在37 ℃的生理温度下是稳定的。用L-精氨酸的D型取代L-精氨酸可提高其血清稳定性。对这种用于生物医学应用的复杂纳米材料平台的进一步初步研究表明，与镁组装的对应物相比，DNA/氨基酸表现出不同的细胞摄取行为。纳米材料主要聚集在细胞膜周围，并可能被用来操纵膜上的分子事件。我们的研究表明，DNA纳米结构的性质可以通过将它们与定制的客体分子复合来调节。本文提出的策略可能是有前途的推进DNA纳米结构的生物医学应用。提出了一种“非正则DNA自组装”策略。阳离子氨基酸可以在无镁体系中组装DNA纳米结构。复合纳米材料具有较高的结构稳定性和血清稳定性。DNA纳米结构可以用定制的客体分子进行工程化以用于多种应用。
Complexing self-assembled DNA nanostructures with various functional guest species is the key to unlocking new and exciting biomedical applications. Cationic guest species not only induce magnesium-free DNA to self-assemble into defined structures but also endow the final complex nanomaterials with new properties. Herein, we propose a novel strategy that employs naturally occurring cationic amino acids to induce DNA self-assembly into defined nanostructures. Natural l-arginine and l-lysine can readily induce the assembly of tile-based DNA nanotubes and DNA origami sheets in a magnesium-free manner. The self-assembly processes are demonstrated to be pH- and concentration-dependent and are achieved at constant temperatures. Moreover, the assembled DNA/amino acid complex nanomaterials are stable at a physiological temperature of 37 °C. Substituting l-arginine with its D form enhances its serum stability. Further preliminary examination of this complex nanomaterial platform for biomedical applications indicates that DNA/amino acids exhibit distinct cellular uptake behaviors compared with their magnesium-assembled counterparts. The nanomaterial mainly clusters around the cell membrane and might be utilized to manipulate molecular events on the membrane. Our study suggests that the properties of DNA nanostructures can be tuned by complexing them with customized guest molecules for a designed application. The strategy proposed herein might be promising to advance the biomedical applications of DNA nanostructures. A “noncanonical DNA self-assembly” strategy is proposed. Cationic amino acids can assemble DNA nanostructures in a magnesium-free system. The complex nanomaterial exhibited high structural and serum stability. DNA nanostructures can be engineered with customized guest molecules for multiple applications.
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