High-efficiency regeneration of Agrobacterium rhizogenes-induced hairy root in apple rootstock Malus baccata (L.) Borkh
High-efficiency regeneration of Agrobacterium rhizogenes-induced hairy root in apple rootstock Malus baccata (L.) Borkh
复制标题
发根农杆菌诱导的苹果砧木Malus baccata (L.) Borkh毛状根的高效再生
DOI：
10.1007/s11240-012-0182-1
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Han, Zhen-Hai1
中科院分区：
文献类型：
--
作者：
Wu, Jiao1, 2;Wang, Yi1;Zhang, Liu-Xia1;Zhang, Xin-Zhong1;Kong, Jin1;Lu, Jiang2;Han, Zhen-Hai1
山荆子因其抗寒性强，在世界寒地被广泛用作砧木。本研究以发根农杆菌8196菌株为材料，建立了高效的发根农杆菌转化体系。浆果。当它们在不含植物生长调节剂的Murashige和Skoog（MS）培养基上孵育时，大约37%的农杆菌感染的外植体产生毛状根。95%的毛状根具有葡萄糖醛酸酶活性.从假定转化的毛状根诱导愈伤组织，随后在培养的4周内观察到芽。采用L9（34）正交试验研究了6-苄基腺嘌呤（BA）、吲哚-3-丁酸（IBA）、噻苯隆（TDZ）和赤霉素3（GA 3）对再生的影响。当愈伤组织在MS +2.0 mg L−1 BA、0.5 mg L−1 IBA、0.3 mg L−1 GA 3和0.5 mg L−1 TDZ的培养基上沿着培养时，约73%的芽再生。此外，毛状根再生体的生根能力也比对照高，且表现出茎缩短、叶片黄化、起皱和丛生等形态畸变。
Malus baccata is widely used as a rootstock in cold regions of the world because of its cold hardiness. In this study, a highly efficient Agrobacterium rhizogenes strain 8196 transformation system was developed using in vitro-derived stem segments of M. baccata. Approximately 37 % agro-infected explants produced hairy roots when they were incubated on Murashige and Skoog (MS) medium without plant growth regulators. A total of 95 % of hairy roots exhibited glucuronidase activity. Calli were induced from putatively-transformed hairy roots, and subsequently shoots were observed within 4 weeks of culture. The influence of 6-benzyladenine (BA), indole-3-butyric acid (IBA), thidiazuron (TDZ), and gibberellic acid 3 (GA3) on regeneration were investigated using an L9 (34) orthogonal experiment. About 73 % of shoots were regenerated when callus was incubated on MS medium along with 2.0 mg L−1 BA, 0.5 mg L−1 IBA, 0.3 mg L−1 GA3, and 0.5 mg L−1 TDZ. Moreover, hairy root regenerants showed higher rooting ability and exhibited morphological aberrations such as shortened stem, etiolated, wrinkled and clustered leaves than those of control.
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影响因子：
44.1
作者：
Y. Wang;J. Wang;D. Luo;J. Jia
通讯作者：
Y. Wang;J. Wang;D. Luo;J. Jia
影响因子：
3
作者：
Chevreau, E.;Dupuis, F.;Loridon, K.
通讯作者：
Loridon, K.
影响因子：
7.2
作者：
Collier, R;Fuchs, B;Taylor, CG
通讯作者：
Taylor, CG
影响因子：
3.2
作者：
Bolar, JP;Norelli, JL;Aldwinckle, HS
通讯作者：
Aldwinckle, HS
DOI：
--
发表时间：
2010
期刊：
Research Journal of Agriculture and Biological Sciences
影响因子：
--
作者：
M. S. A. Rayya;N. Kassem;E. A. Ali
通讯作者：
M. S. A. Rayya;N. Kassem;E. A. Ali