Highly scalable multichannel mesh electronics for stable chronic brain electrophysiology.
Highly scalable multichannel mesh electronics for stable chronic brain electrophysiology.
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DOI：
10.1073/pnas.1717695114
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发表时间：
2017-11-21
影响因子：
11.1
通讯作者：
Lieber CM
中科院分区：
文献类型：
--
作者：
Fu TM;Hong G;Viveros RD;Zhou T;Lieber CM
植入式电探针已经在神经科学和神经系统疾病的治疗方面取得了重大进展，但却无法稳定地追踪大量对大脑功能至关重要的单个神经元的长期进化。在这里，我们展示了一种可扩展的方案，用于高度复用的网状电子探针，克服了这一长期存在的挑战。我们通过制造具有大孔神经网络结构和与大脑相当的灵活性的32至128通道探针来说明该方案。在植入啮齿类动物大脑后，我们展示了4个月内多个大脑区域单神经元水平稳定的128通道慢性记录。这些可扩展的网状电子探针代表了一个理想的平台，用于绘制、跟踪和调节与学习、衰老和神经退行性疾病相关的单神经元水平回路变化。植入式电探针在神经科学、脑机接口和神经系统疾病治疗方面取得了进展，但它们在几个关键方面仍然有限。理想情况下，电探针应该能够记录多个局部电路中的大量神经元，重要的是，能够在整个研究过程中稳定地跟踪这些神经元的进化。基于微加工的硅探针可以实现大规模、高密度的记录，但由于与大脑的机械和结构不匹配，面临慢性胶质细胞增生和不稳定性的挑战。另一方面，超柔性网状电子设备在单个神经元水平上表现出可忽略的慢性免疫反应和稳定的长期大脑监测，尽管迄今为止，它仅限于16个通道。在这里，我们提出了一种可扩展的方案，用于高度复用的网状电子探针，以弥合可扩展性和灵活性之间的差距，其中每个探针实现32至128个通道，同时保持关键的类脑结构和力学。将这种网格设计与多位点注射相结合，我们在清醒的受限小鼠中展示了稳定的128通道局部场电位和来自多个大脑区域的单单元记录，超过4个月。此外，新集成的网格用于验证自由行为小鼠的稳定慢性记录。这种网状电子学的可扩展方案以及证明的长期稳定性代表了实现理想植入式电探针的重要进展，允许映射和跟踪与学习，衰老和神经退行性疾病相关的单神经元水平电路变化。
Implantable electrical probes have led to fundamental neuroscience advances and treatment of neurological diseases, yet are unable to stably track the long-term evolution of large numbers of individual neurons critical to brain functions. Here, we demonstrate a scalable scheme for highly multiplexed mesh electronics probes that overcomes this long-standing challenge. We illustrate this scheme through fabrication of 32 to 128 channel probes with macroporous neural network-like structure and flexibility comparable to the brain. Following implantation into rodent brains, we demonstrate chronic 128-channel recordings with single-neuron-level stability from multiple brain regions over 4 mo. These scalable mesh electronics probes represent an ideal platform for mapping, tracking, and modulating the single-neuron-level circuit changes associated with learning, aging, and neurodegenerative diseases. Implantable electrical probes have led to advances in neuroscience, brain−machine interfaces, and treatment of neurological diseases, yet they remain limited in several key aspects. Ideally, an electrical probe should be capable of recording from large numbers of neurons across multiple local circuits and, importantly, allow stable tracking of the evolution of these neurons over the entire course of study. Silicon probes based on microfabrication can yield large-scale, high-density recording but face challenges of chronic gliosis and instability due to mechanical and structural mismatch with the brain. Ultraflexible mesh electronics, on the other hand, have demonstrated negligible chronic immune response and stable long-term brain monitoring at single-neuron level, although, to date, it has been limited to 16 channels. Here, we present a scalable scheme for highly multiplexed mesh electronics probes to bridge the gap between scalability and flexibility, where 32 to 128 channels per probe were implemented while the crucial brain-like structure and mechanics were maintained. Combining this mesh design with multisite injection, we demonstrate stable 128-channel local field potential and single-unit recordings from multiple brain regions in awake restrained mice over 4 mo. In addition, the newly integrated mesh is used to validate stable chronic recordings in freely behaving mice. This scalable scheme for mesh electronics together with demonstrated long-term stability represent important progress toward the realization of ideal implantable electrical probes allowing for mapping and tracking single-neuron level circuit changes associated with learning, aging, and neurodegenerative diseases.
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影响因子：
38.3
作者：
Liu J;Fu TM;Cheng Z;Hong G;Zhou T;Jin L;Duvvuri M;Jiang Z;Kruskal P;Xie C;Suo Z;Fang Y;Lieber CM
通讯作者：
Lieber CM
影响因子：
41.2
作者：
通讯作者：
--
细胞外田地和电流的起源-EEG，ECOG，LFP和尖峰。
DOI：
10.1038/nrn3241
发表时间：
2012-05-18
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
Buzsáki G;Anastassiou CA;Koch C
通讯作者：
Koch C
提高神经元群体记录的数据质量。
影响因子：
25
作者：
Harris KD;Quiroga RQ;Freeman J;Smith SL
通讯作者：
Smith SL
具有多功能电子器件的工程混合心脏贴片，用于在线监测和调节组织功能。
影响因子：
41.2
作者：
Feiner R;Engel L;Fleischer S;Malki M;Gal I;Shapira A;Shacham-Diamand Y;Dvir T
通讯作者：
Dvir T