Effect of antibiotic treatment on microbial composition and expression of antimicrobial peptides and cytokines in the chick cecum.
Effect of antibiotic treatment on microbial composition and expression of antimicrobial peptides and cytokines in the chick cecum.
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DOI：
10.1016/j.psj.2020.03.016
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发表时间：
2020-07
期刊：
影响因子：
4.4
通讯作者：
Yoshimura Y
中科院分区：
文献类型：
--
作者：
Terada T;Nii T;Isobe N;Yoshimura Y
本研究的目的是确认鸡盲肠中先天免疫分子的表达是否与抗生素治疗调节的肠道微生物组组成的变化相关。每天给肉鸡施用抗生素（青霉素和链霉素），并在第7天和第14天使用实时PCR和免疫组织化学检查鸡盲肠中的微生物群的组成、先天免疫分子的表达和抗菌肽的定位。口服抗生素导致肠杆菌科的相对频率增加，而一些革兰氏阴性菌（Barnesiellaceae）和革兰氏阳性菌（梭菌科和丹毒丝菌科）家族的相对频率减少。免疫分子的基因表达水平，包括4种Toll样受体（TLR）（TLR 2、4、5和21）、炎症相关细胞因子（IL-1β、TGFβ3、TGFβ4和IL-8）和抗菌肽（禽β-防御素和凯萨林菌素），在第7天显示出抗生素治疗降低的趋势。然而，TLR 21和一些细胞因子（IL-1β、TGFβ3和IL-8）的表达水平在第14天时在盲肠或盲肠扁桃体中比在对照组中更高。禽β-防御素2和cathelicidin 1免疫反应蛋白定位于固有层的白细胞样细胞中，呈小岛状聚集。我们的结论是先天免疫分子，包括TLR，炎症相关的细胞因子和抗菌肽的表达，在盲肠中的改变与腔菌群的密度或组成的变化在鸡的生命的早期阶段。
The aim of this study was to confirm whether the expression of innate immune molecules in the chick cecum is altered in association with changes in the composition of the intestinal microbiome that are regulated by treatment with antibiotics. Broiler chicks were administered with antibiotics (penicillin and streptomycin) daily, and the composition of the microbiota, expression of innate immune molecules, and localization of antimicrobial peptides in the chick cecum were examined at day 7 and day 14 using real-time PCR and immunohistochemistry. The oral administration of antibiotics caused an increase in the relative frequency of the Enterobacteriaceae family and a decrease in some gram-negative (Barnesiellaceae) and gram-positive bacterial (Clostridiaceae and Erysipelotrichaceae) families. The gene expression levels of immune molecules, including 4 Toll-like receptors (TLR) (TLR 2, 4, 5, and 21), inflammation-related cytokines (IL-1β, TGFβ3, TGFβ4, and IL-8), and antimicrobial peptides (avian β-defensins and cathelicidins) showed a tendency to decrease with antibiotic treatment at day 7. However, expression levels of TLR21 and some cytokines (IL-1β, TGFβ3, and IL-8) were higher in the cecum or cecal tonsils of the antibiotic-treated group than in those of the control at day 14. The immunoreactive avian β-defensin 2 and cathelicidin 1 proteins were localized in the leukocyte-like cells in the lamina propria, and they were aggregated in the form of small islands. We conclude that the expression of innate immune molecules, including TLR, inflammation-related cytokines, and antimicrobial peptides, in the cecum are altered in association with changes in the density or composition of the luminal microbiota during the early phase of life in chicks.
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影响因子：
2.9
作者：
Cuperus, Tryntsje;van Dijk, Albert;Haagsman, Henk P.
通讯作者：
Haagsman, Henk P.
影响因子：
3.8
作者：
Sonoda, Yuka;Mageed, Ahmad M. Abdel;Yoshimura, Yukinori
通讯作者：
Yoshimura, Yukinori
影响因子：
1.6
作者：
Inoue, Ryo;Yajima, Takaji;Tsukahara, Takamitsu
通讯作者：
Tsukahara, Takamitsu
影响因子：
3.3
作者：
Taha-Abdelaziz, Khaled;Alkie, Tamiru Negash;Sharif, Shayan
通讯作者：
Sharif, Shayan
DOI：
10.2141/jpsa.0190098
发表时间：
2020-10-25
期刊：
The journal of poultry science
影响因子：
--
作者：
Terada T;Nii T;Isobe N;Yoshimura Y
通讯作者：
Yoshimura Y