Detection of bacterial pathogens in aquaculture samples by DNA microarray analysis
Detection of bacterial pathogens in aquaculture samples by DNA microarray analysis
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通过 DNA 微阵列分析检测水产养殖样品中的细菌病原体
DOI：
10.1016/j.aquaculture.2012.01.009
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发表时间：
2012-03
期刊：
影响因子：
4.5
通讯作者：
Li, Ming-Yun
中科院分区：
文献类型：
--
作者：
Shi, Yu-Hong;Chen, Jiong;Li, Chang-Hong;Lu, Xin-Jiang;Zhang, De-Ming;Li, Hao-Yan;Zhao, Zhi-Xian;Li, Ming-Yun
快速、灵敏和特异的病原体检测对于有效控制水产养殖中的疾病具有重要意义。然而，使用传统的微生物方法进行病原体检测往往受到完成检测所需时间的限制。本研究旨在建立一种检测养殖水产动物中8种病原菌的DNA芯片技术，即哈维氏弧菌、溶藻弧菌、副溶血弧菌、鳗弧菌、霍乱弧菌、轮状诺卡氏菌、嗜水气单胞菌和新链球菌。根据细菌16S rRNA基因可变区和gryB序列设计探针，与25种细菌类型培养物杂交，最终筛选出23个探针。检测健康鱼血中鳗弧菌的芯片灵敏度为≥100CFU/mL。应用基因芯片、常规培养和16S rRNA基因测序方法对21份病原细菌进行盲法分析，结果与常规培养和16S rRNA基因测序结果一致。本研究的结果表明，所描述的微阵列方法适用于水生动物主要潜在病原体的初步诊断或确认。
Rapid, sensitive, and specific detection of pathogens is important for effective disease control in aquaculture. However, the use of conventional microbiological methods for pathogen detection is often limited by the time required to complete the assays. The aim of this study was to develop a DNA microarray assay to detect 8 species of pathogenic bacteria, i.e., Vibrio harveyi, V. alginolyticus, V. parahaemolyticus, V. anguillarum, V. cholerae, Nocardia seriolae, Aeromonas hydrophila, and Streptococcus iniae, in cultured aquatic animals. Probes were designed according to a variable region of the bacterial 16S rRNA gene and gryB sequences, and 23 probes were finally selected by hybridization with 25 bacterial type cultures. The microarray sensitivity for V. anguillarum detection in healthy fish blood was ≥100CFU/mL. Pathogenic bacteria in blood samples from 21 diseased aquacultural samples were analyzed in a blinded fashion by using the microarray, conventional culture, and 16S rRNA gene sequencing methods; the results of the microarray assay agreed well with those of the conventional culture and 16S rRNA gene sequencing methods. The results of this study show that the microarray method described is suitable for preliminary diagnoses or for confirmation of the main potential pathogens in aquatic animals.
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影响因子：
9.4
作者：
Zhu, Lingxiang;Jiang, Guanglu;Guo, Yong
通讯作者：
Guo, Yong
影响因子：
2.5
作者：
G.-L. Wang;S.-P. Yuan;S. Jin
通讯作者：
G.-L. Wang;S.-P. Yuan;S. Jin
影响因子：
4.1
作者：
Tomioka, K;Peredelchuk, M;Duncan, R
通讯作者：
Duncan, R
影响因子：
4.5
作者：
L. Phuoc;M. Corteel;N. C. Thanh;H. Nauwynck;M. Pensaert;V. Alday‐Sanz;W. Broeck;P. Sorgeloos;P. Bossier
通讯作者：
L. Phuoc;M. Corteel;N. C. Thanh;H. Nauwynck;M. Pensaert;V. Alday‐Sanz;W. Broeck;P. Sorgeloos;P. Bossier
影响因子：
3.4
作者：
Wen Wang
通讯作者：
Wen Wang