Development of molecular assays for the rapid and cost-effective determination of fluoroquinolone, macrolide and lincosamide susceptibility of Mycoplasma synoviae isolates.
Development of molecular assays for the rapid and cost-effective determination of fluoroquinolone, macrolide and lincosamide susceptibility of Mycoplasma synoviae isolates.
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DOI：
10.1371/journal.pone.0241647
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Gyuranecz M
中科院分区：
文献类型：
--
作者：
Bekő K;Kreizinger Z;Yvon C;Saller O;Catania S;Feberwee A;Gyuranecz M
滑膜支原体感染在世界范围内都有发生，由于传染性滑膜炎、呼吸系统疾病和蛋壳顶端异常，给鸡和火鸡产业造成了相当大的经济损失。针对滑膜分枝杆菌感染的控制方案是基于根除、疫苗接种和抗菌药物治疗。通过在治疗前确定抗生素敏感性，可以大大提高抗生素的谨慎使用。然而，传统的肉汤或琼脂微量稀释是非常劳动密集型和耗时的方法。因此，为了更有效地指导抗生素治疗，对快速抗菌药物敏感性试验的需求日益增加。本研究的目的是建立错配扩增突变测定法（MAMAs）来检测滑膜分枝杆菌的耐药性相关突变。使用先前确定的最低抑制浓度（mic）和全基因组（n = 92）的滑膜分枝杆菌菌株进行目标选择和试验规范。为了评估开发的检测方法，本研究还包括20份临床样本和另外20份来自这些样本的滑膜分枝杆菌分离株。采用肉汤微量稀释法测定20株菌株的MIC值。设计了5种MAMAs来检测氟喹诺酮类药物的mic升高，而开发了3种MAMAs来检测大环内酯类药物和林可霉素的敏感性降低。MAMA试验的灵敏度在102−104模板拷贝数/反应之间变化，取决于测定。临床样本显示与每个病例中相应标本衍生的滑膜分枝杆菌分离株相同的基因型呼叫。支持常规体外敏感性试验的结果，我们的方法为诊断提供了一种可行的工具。快速，稳健性和成本效益是开发的分析的强大优势。支持谨慎使用抗生素而不是经验性治疗，使用这种方法可以显著减少滑膜分枝杆菌对家禽业的经济影响，并减少与细菌耐药性相关的公共卫生问题。
Mycoplasma synoviae infection occurs worldwide, leading to considerable economic losses in the chicken and turkey industry due to infectious synovitis, respiratory diseases and eggshell apex abnormalities. Control programs against M. synoviae infection are based on eradication, vaccination and medication with antimicrobial agents. Prudent use of antibiotics can be improved greatly by the determination of antibiotic susceptibility prior to the treatment. However, the conventional broth or agar microdilution is very labor-intensive and time-consuming method. Thus, there is an increasing need for rapid antimicrobial susceptibility tests in order to guide antibiotic therapy more effectively. The aim of this study was to develop mismatch amplification mutation assays (MAMAs) to detect resistance-associated mutations in M. synoviae. M. synoviae strains with previously determined minimal inhibitory concentrations (MICs) and whole genomes (n = 92) were used for target selection and assay specification. For the evaluation of the developed assays, 20 clinical samples and an additional 20 M. synoviae isolates derived from these specimens were also included in this study. MIC values of these 20 isolates were determined by broth microdilution method. Five MAMAs were designed to identify elevated MICs of fluoroquinolones, while three MAMAs were developed to detect decreased susceptibility to macrolides and lincomycin. The sensitivity of the MAMA tests varied between 102−104 template copy number/reaction depending on the assay. Clinical samples showed identical genotype calls with the M. synoviae isolates derived from the corresponding specimens in each case. Supporting the results of conventional in vitro sensitivity tests, our approach provides a feasible tool for diagnostics. Rapidity, robustness and cost-effectiveness are powerful advantages of the developed assays. Supporting prudent antibiotic usage instead of empirical treatment, the use of this method can reduce significantly the economic impact of M. synoviae in the poultry industry and decrease bacterial resistance-related public health concerns.
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影响因子：
1.4
作者：
Lauerman, LH;Chilina, AR;Johansen, D
通讯作者：
Johansen, D
DOI：
10.1093/bioinformatics/bts199
发表时间：
2012-06-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Kearse M;Moir R;Wilson A;Stones-Havas S;Cheung M;Sturrock S;Buxton S;Cooper A;Markowitz S;Duran C;Thierer T;Ashton B;Meintjes P;Drummond A
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影响因子：
11.5
作者：
Piddock, LJV
通讯作者：
Piddock, LJV
影响因子：
3.7
作者：
Catania, Salvatore;Bottinelli, Marco;Vicenzoni, Gaddo
通讯作者：
Vicenzoni, Gaddo
影响因子：
2.6
作者：
Kreizinger Z;Grózner D;Sulyok KM;Nilsson K;Hrivnák V;Benčina D;Gyuranecz M
通讯作者：
Gyuranecz M