Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis.
Norovirus RNA in serum associated with increased fecal viral load in children: Detection, quantification and molecular analysis.
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DOI：
10.1371/journal.pone.0199763
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Gabbay YB
中科院分区：
文献类型：
--
作者：
Reymão TKA;Fumian TM;Justino MCA;Hernandez JM;Bandeira RS;Lucena MSS;Teixeira DM;Farias FP;Silva LD;Linhares AC;Gabbay YB
在世界范围内，诺如病毒 (NoV) 是急性胃肠炎 (AGE) 的主要原因，每 3 年就会发生一次大流行，每年造成超过 200,000 人死亡，其中大多数是发展中国家的儿童。我们调查了巴西帕拉州贝伦市因 AGE 住院的儿童中 NoV 的发病率，并将其血液和粪便中的病毒 RNA 水平与临床严重程度相关联。为此，从 2012 年 3 月至 2015 年 6 月期间，从 445 名 9 岁以下儿童患者中采集了配对粪便和血清样本。使用酶联免疫吸附测定 (EIA) 检测粪便中的 NoV，并使用逆转录定量 PCR (RT-qPCR) 定量血清（RNA 血症）和阳性粪便中的 NoV RNA 水平。阳性样本的特征在于病毒基因组的部分 ORF1/2 区域序列。在 24.3% (108/445) 的粪便样本中检测到 NoV 抗原，20.4% (22/108) 中还存在 RNA 血症。 RNA 血症和高粪便病毒载量（> 107 个基因组拷贝/克粪便）与较长的住院时间相关。流行的基因型是 GII.4 Sydney_2012 (71.6%-58/81) 和 New Orleans_2009 (6.2%-5/81) 变体。还检测到了其他 8 个属于 GII 的基因型，其中 4 个是重组菌株。所有血清均被定性为 GII.4，并与其粪便具有 100% 的相似性。结果表明，NoV 向血流传播并不少见，可能与粪便病毒载量增加和疾病严重程度有关。
Worldwide, norovirus (NoV) is a major cause of acute gastroenteritis (AGE) responsible for pandemics every ~3 years, and over 200,000 deaths per year, with the majority in children from developing countries. We investigate the incidence of NoV in children hospitalized with AGE from Belém, Pará, Brazil, and also correlated viral RNA levels in their blood and stool with clinical severity. For this purpose, paired stool and serum samples were collected from 445 pediatric patients, ≤9 years between March 2012 and June 2015. Enzyme-linked immunosorbent assay (EIA) was used to detect NoV in stool and reverse transcription quantitative PCR (RT-qPCR) used to quantify NoV RNA levels in sera (RNAemia) and in the positive stool. Positives samples were characterized by the partial ORF1/2 region sequence of viral genome. NoV antigen was detected in 24.3% (108/445) of stool samples, with RNAemia also present in 20.4% (22/108). RNAemia and a high stool viral load (>107 genome copies/gram of faeces) were associated with longer hospitalizations. The prevalent genotypes were GII.4 Sydney_2012 (71.6%-58/81) and New Orleans_2009 (6.2%-5/81) variants. Eight other genotypes belonging to GII were detected and four of them were recombinant strains. All sera were characterized as GII.4 and shared 100% similarity with their stool. The results suggest that the dissemination of NoV to the blood stream is not uncommon and may be related to increased faecal viral loads and disease severity.
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统一的诺如病毒术语和基因分型的提案。
影响因子：
2.7
作者：
Kroneman A;Vega E;Vennema H;Vinjé J;White PA;Hansman G;Green K;Martella V;Katayama K;Koopmans M
通讯作者：
Koopmans M
影响因子：
3.7
作者：
Hernandez JM;Silva LD;Junior ECS;Bandeira RS;Rodrigues EAM;Lucena MSS;Costa STP;Gabbay YB
通讯作者：
Gabbay YB
影响因子：
10.7
作者：
Minh BQ;Nguyen MA;von Haeseler A
通讯作者：
von Haeseler A
影响因子：
8.8
作者：
Huhti, Leena;Hemming-Harlo, Maria;Vesikari, Timo
通讯作者：
Vesikari, Timo
DOI：
10.1016/j.diagmicrobio.2011.08.023
发表时间：
2011-12-01
影响因子：
2.9
作者：
Monteiro Siqueira, Jones Anderson;Linhares, Alexandre da Costa;Gabbay, Yvone Benchimol
通讯作者：
Gabbay, Yvone Benchimol