Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy.
Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy.
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DOI：
10.1371/journal.pone.0132467
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Mathias CB
中科院分区：
文献类型：
--
作者：
Kinney SR;Carlson L;Ser-Dolansky J;Thompson C;Shah S;Gambrah A;Xing W;Schneider SS;Mathias CB
IgE抗体和肥大细胞在建立对食物抗原的过敏反应中起关键作用。姜黄素是咖喱香料姜黄的活性成分，具有抗炎特性，因此可能具有在过敏反应期间调节Th 2细胞和粘膜肥大细胞功能的能力。我们使用卵清蛋白（OVA）诱导的肠道过敏反应的小鼠模型评估了在口服过敏原暴露期间摄入姜黄素是否可以调节食物过敏的发展。在此，我们证明了在口服OVA暴露期间频繁摄入姜黄素抑制了OVA激发的过敏小鼠中肥大细胞增多症和肠道过敏反应的发展。胃内（i.g.）致敏的BALB/c小鼠暴露于OVA诱导了强烈的IgE介导的应答，伴随着OVA-IgE水平升高、肠肥大细胞增多症、血清mMCP-1升高和急性腹泻。相反，在整个实验方案中暴露于口服姜黄素的小鼠似乎是正常的，并且没有表现出强烈的过敏性腹泻或OVA-IgE和肠肥大细胞扩增和活化的显著增强。此外，过敏性腹泻，肥大细胞的活化和扩增，和Th 2反应也受到抑制，在暴露于姜黄素的小鼠在单独的OVA-挑战阶段，尽管存在水平升高的OVA-IgE，表明姜黄素可能有一个直接的抑制作用，肠道肥大细胞的活化和逆转过敏原致敏的个人的食物过敏症状。姜黄素减弱了过继转移的骨髓源性肥大细胞（BMMC）的扩增，并在细胞培养期间抑制其存活和活化，这一点得到了证实。最后，姜黄素对肠道过敏反应的抑制与姜黄素处理的过敏小鼠中NF-κB活化的抑制直接相关，并且姜黄素抑制BMMCs中NF-κB的p65亚基的磷酸化。总之，我们的数据表明，姜黄素在对食物抗原的过敏反应中具有保护作用，这表明经常摄入这种香料可能会调节易感个体的疾病结果。
IgE antibodies and mast cells play critical roles in the establishment of allergic responses to food antigens. Curcumin, the active ingredient of the curry spice turmeric, has anti-inflammatory properties, and thus may have the capacity to regulate Th2 cells and mucosal mast cell function during allergic responses. We assessed whether curcumin ingestion during oral allergen exposure can modulate the development of food allergy using a murine model of ovalbumin (OVA)-induced intestinal anaphylaxis. Herein, we demonstrate that frequent ingestion of curcumin during oral OVA exposure inhibits the development of mastocytosis and intestinal anaphylaxis in OVA-challenged allergic mice. Intragastric (i.g.) exposure to OVA in sensitized BALB/c mice induced a robust IgE-mediated response accompanied by enhanced OVA-IgE levels, intestinal mastocytosis, elevated serum mMCP-1, and acute diarrhea. In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation. Furthermore, allergic diarrhea, mast cell activation and expansion, and Th2 responses were also suppressed in mice exposed to curcumin during the OVA-challenge phase alone, despite the presence of elevated levels of OVA-IgE, suggesting that curcumin may have a direct suppressive effect on intestinal mast cell activation and reverse food allergy symptoms in allergen-sensitized individuals. This was confirmed by observations that curcumin attenuated the expansion of both adoptively transferred bone marrow-derived mast cells (BMMCs), and inhibited their survival and activation during cell culture. Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs. In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.
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DOI：
10.4049/jimmunol.1303026
发表时间：
2014-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Johnston LK;Chien KB;Bryce PJ
通讯作者：
Bryce PJ
DOI：
10.1186/1476-7961-5-1
发表时间：
2007-01-25
期刊：
Clinical and molecular allergy : CMA
影响因子：
--
作者：
Kurup, Viswanath P;Barrios, Christy S;Raju, Raghavan;Johnson, Bryon D;Levy, Michael B;Fink, Jordan N
通讯作者：
Fink, Jordan N
影响因子：
8.7
作者：
Burton OT;Oettgen HC
通讯作者：
Oettgen HC
影响因子：
5.4
作者：
Cong, Yingzi;Wang, Lanfang;Elson, Charles O.
通讯作者：
Elson, Charles O.
影响因子：
7.7
作者：
Karaman, Meral;Ayyildiz, Zeynep Arikan;Karaman, Ozkan
通讯作者：
Karaman, Ozkan