Neurite extension of chicken peripheral nervous system neurons on fibronectin: relative importance of specific adhesion sites in the central cell-binding domain and the alternatively spliced type III connecting segment.
Neurite extension of chicken peripheral nervous system neurons on fibronectin: relative importance of specific adhesion sites in the central cell-binding domain and the alternatively spliced type III connecting segment.
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DOI：
10.1083/jcb.106.4.1289
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发表时间：
1988-04
期刊：
影响因子：
--
通讯作者：
Yamada KM
中科院分区：
文献类型：
--
作者：
Humphries MJ;Akiyama SK;Komoriya A;Olden K;Yamada KM
纤连蛋白含有至少两个支持细胞粘附的结构域。一个是中央细胞结合结构域，其被多种细胞类型（包括成纤维细胞）识别。第二，最初确定其支持黑色素瘤细胞粘附的能力，位于可变剪接的III型连接段（IIICS）。使用特定的粘合剂配体和抑制性探针，我们已经研究了这些领域中的每一个在纤维连接蛋白介导的神经元从鸡胚背根和交感神经节的轴突延伸的作用。在研究中，使用神经节，无论是fl 3，一个75 kD的胰蛋白酶片段的人血浆纤连蛋白含有中央细胞结合结构域，和CS 1-IgG，合成肽-IgG共轭物含有的主要细胞粘附位点的IIICS，支持神经突生长后吸附到基板上。f13和CS 1- IgG的最大活性分别为完整纤连蛋白的45-55%和25-30%。用f13和CS 1-IgG共包被基质产生了神经突生长的附加刺激，其程度接近用纤连蛋白获得的程度。用胰蛋白酶解离背根神经节分离纯化的神经元细胞制备物获得了类似的结果。在补充研究中，阻断中央细胞结合结构域（mAb 333，抗粘附单克隆抗体）或IIICS（CS 1肽）的粘附功能，分别导致纤连蛋白介导的神经突生长减少约60%或30%。当组合测试时，mAb 333和CS 1的抑制活性是相加的。从这些结果中，我们得出结论，从周围神经系统的神经元可以延长神经突起的中央细胞结合域和IIICS区域的纤连蛋白，因此，这些细胞是第一个正常的，胚胎细胞类型显示坚持IIICS。这些结果表明，时空波动的替代mRNA剪接的纤连蛋白的IIICS区域可能是重要的调节细胞粘附事件在周围神经系统的发展。
Fibronectin contains at least two domains that support cell adhesion. One is the central cell-binding domain that is recognized by a variety of cell types, including fibroblasts. The second, originally identified by its ability to support melanoma cell adhesion, is located in the alternatively spliced type III connecting segment (IIICS). Using specific adhesive ligands and inhibitory probes, we have examined the role of each of these domains in fibronectin-mediated neurite extension of neurons from chick embryo dorsal root and sympathetic ganglia. In studies using explanted ganglia, both fl3, a 75-kD tryptic fragment of human plasma fibronectin containing the central cell-binding domain, and CS1-IgG, a synthetic peptide-IgG conjugate containing the principal cell adhesion site from the IIICS, supported neurite outgrowth after adsorption onto the substrate. The maximal activities of fl3 and CSl- IgG were 45-55% and 25-30% that of intact fibronectin, respectively. Co- coating of the substrate with f13 and CS1-IgG produced an additive stimulation of neurite outgrowth, the extent of which approached that obtained with fibronectin. Similar results were obtained with purified neuronal cell preparations isolated by tryptic dissociation of dorsal root ganglia. In complementary studies, blockage of the adhesive function of either the central cell-binding domain (with mAb 333, an antiadhesive monoclonal antibody) or the IIICS (with CS1 peptide), resulted in approximately 60 or 30% reduction in fibronectin-mediated neurite outgrowth, respectively. When tested in combination, the inhibitory activities of mAb 333 and CSl were additive. From these results, we conclude that neurons from the peripheral nervous system can extend neurites on both the central cell-binding domain and the IIICS region of fibronectin, and that these cells are therefore the first normal, embryonic cell type shown to adhere to the IIICS. These results suggest that spatiotemporal fluctuations in the alternative mRNA splicing of the IIICS region of fibronectin may be important in regulation of cell adhesive events during development of the peripheral nervous system.
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DOI：
10.1073/pnas.80.12.3850
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CORNBROOKS, CJ;CAREY, DJ;BUNGE, RP
通讯作者：
BUNGE, RP
DOI：
10.1083/jcb.101.6.2134
发表时间：
1985-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Horwitz A;Duggan K;Greggs R;Decker C;Buck C
通讯作者：
Buck C
影响因子：
2.5
作者：
FAIVREBAUMAN, A;PUYMIRAT, J;TIXIERVIDAL, A
通讯作者：
TIXIERVIDAL, A
影响因子：
56.9
作者：
GOODMAN, CS;BASTIANI, MJ;THOMAS, JB
通讯作者：
THOMAS, JB
影响因子：
2.7
作者：
HAMMARBACK, JA;LETOURNEAU, PC
通讯作者：
LETOURNEAU, PC