Combinatorial Glycomic Analyses to Direct CAZyme Discovery for the Tailored Degradation of Canola Meal Non-Starch Dietary Polysaccharides.
Combinatorial Glycomic Analyses to Direct CAZyme Discovery for the Tailored Degradation of Canola Meal Non-Starch Dietary Polysaccharides.
复制标题
直接发现CAZyme对菜籽粕非淀粉膳食多糖量身定制降解的组合糖学分析。
DOI：
10.3390/microorganisms8121888
复制
发表时间：
2020-11-29
期刊：
影响因子：
4.5
通讯作者：
Abbott DW
中科院分区：
文献类型：
--
作者：
Low KE;Xing X;Moote PE;Inglis GD;Venketachalam S;Hahn MG;King ML;Tétard-Jones CY;Jones DR;Willats WGT;Slominski BA;Abbott DW
芥花籽粕（CM）是芥花籽油提取的富含蛋白质的副产品，已显示出作为家禽饮食中的替代饲料和蛋白质补充剂的前景，但由于植物细胞壁纤维，特别是非淀粉多糖（NSP）和木质素的丰富，其使用受到限制。添加外源酶促进鸡对CM NSP的消化，有可能提高CM的代谢能。我们从连续流动微型生物反应器系统中分离鸡盲肠细菌，并选择具有代谢CM NSP能力的细菌。在鉴定的100株分离株中，拟杆菌属（Bacteroides spp.）和肠球菌属的细菌。是拥有这些能力的最常见物种。为了鉴定特异性消化CM NSP的酶，我们使用了糖组学技术的组合，包括植物细胞壁组分的酶联免疫吸附测定表征，糖苷键分析，CM NSP及其级分的甲基化-GC-MS分析，使用补充有CM NSP的基本培养基的细菌生长曲线，以及高效CM NSP利用细菌的细菌基因组的测序和从头注释。SACHARIS流水线用于选择用于重组生产和表征的植物细胞壁活性酶。这种方法代表了一个多学科的创新平台，以生物展望来自草食性和杂食性动物肠道微生物群的内源性CAZymes，其适用于各种应用和膳食多糖。
Canola meal (CM), the protein-rich by-product of canola oil extraction, has shown promise as an alternative feedstuff and protein supplement in poultry diets, yet its use has been limited due to the abundance of plant cell wall fibre, specifically non-starch polysaccharides (NSP) and lignin. The addition of exogenous enzymes to promote the digestion of CM NSP in chickens has potential to increase the metabolizable energy of CM. We isolated chicken cecal bacteria from a continuous-flow mini-bioreactor system and selected for those with the ability to metabolize CM NSP. Of 100 isolates identified, Bacteroides spp. and Enterococcus spp. were the most common species with these capabilities. To identify enzymes specifically for the digestion of CM NSP, we used a combination of glycomics techniques, including enzyme-linked immunosorbent assay characterization of the plant cell wall fractions, glycosidic linkage analysis (methylation-GC-MS analysis) of CM NSP and their fractions, bacterial growth profiles using minimal media supplemented with CM NSP, and the sequencing and de novo annotation of bacterial genomes of high-efficiency CM NSP utilizing bacteria. The SACCHARIS pipeline was used to select plant cell wall active enzymes for recombinant production and characterization. This approach represents a multidisciplinary innovation platform to bioprospect endogenous CAZymes from the intestinal microbiota of herbivorous and omnivorous animals which is adaptable to a variety of applications and dietary polysaccharides.
登录
查看更多内容
抗生素替代品：畜牧业中抗生素的替代品？
影响因子：
5.2
作者：
Cheng G;Hao H;Xie S;Wang X;Dai M;Huang L;Yuan Z
通讯作者：
Yuan Z
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
DOI：
10.1007/978-1-4939-6361-4_18
发表时间：
2016-01-01
期刊：
CLOSTRIDIUM DIFFICILE: METHODS AND PROTOCOLS, 2ND EDITION
影响因子：
--
作者：
Auchtung, Jennifer M.;Robinson, Catherine D.;Britton, Robert A.
通讯作者：
Britton, Robert A.
影响因子：
11.2
作者：
Ghosh, P;Ghosal, P;Ray, B
通讯作者：
Ray, B