Role of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) fed arachidonic acid
Role of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) fed arachidonic acid
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环氧合酶介导的代谢物在饲喂花生四烯酸的草鱼幼鱼脂质代谢和一些免疫相关基因表达中的作用
DOI：
10.1007/s10695-016-0326-z
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发表时间：
2017-06
影响因子：
2.9
通讯作者：
Jin Ai
中科院分区：
文献类型：
--
作者：
Tian Jing-Jing;Lei Cai-Xia;Ji Hong;Jin Ai
环氧合酶（考克斯）催化花生四烯酸（ARA）转化为胡萝卜素，其代谢产物在哺乳动物的脂质代谢和免疫调节中起重要作用。然而，这种作用的考克斯在鱼类仍然是未知的。本研究设计了三种半纯化饲料，即不含ARA（对照）、ARA和ARA +乙酰水杨酸（阿萨;一种考克斯抑制剂），并将其用于饲养草鱼（27.65 ± 3.05 g）8周。结果表明，日粮中添加ARA显著提高了肝胰腺、肌肉和肾脏中ARA的含量（P< 0.05），而日粮中添加阿萨则降低了这种增加。ARA组肝胰腺前列腺素E2含量升高，阿萨可抑制其升高（P< 0.05）。ARA降低肝胰腺脂质含量，ASA使肝胰腺脂质含量恢复到显著水平（P< 0.05）。ARA显著降低肝胰腺脂肪酸合成酶和硬脂酰辅酶A去饱和酶mRNA的表达水平（P< 0.05）。但阿萨对上述基因的表达无明显影响（P> 0.05）。有趣的是，ARA显著增强了过氧化物酶体增殖物激活受体α基因的表达水平，而阿萨则减弱了这种表达水平（P< 0.05）。ARA可显著增强肾组织MyD 88 mRNA的表达，阿萨降低肾组织Toll样受体22和MyD 88 mRNA的表达（P< 0.05）。综上所述，我们的研究结果表明，考克斯代谢产物在抑制草鱼肝胰腺脂肪积累中起重要作用，并且基因表达的调节促进了脂肪催化而不是脂肪生成活性。此外，这些类花生酸可能参与肾脏免疫相关基因的上调。
Cyclooxygenase (COX) catalyzes the conversion of arachidonic acid (ARA) to prostaglandins, and COX-mediated metabolites play important roles in the regulation of lipid metabolism and immunity in mammals. However, such roles of COX in fish remain largely unknown. In this study, we designed three semi-purified diets, namely ARA-free (control), ARA, and ARA + acetylsalicylic acid (ASA; a COX inhibitor), and used them to feed grass carp (27.65 ± 3.05 g) for 8 weeks. The results showed that dietary ARA significantly increased the amount of ARA in the hepatopancreas, muscle, and kidney (P< 0.05), whereas this increase was reduced by dietary ASA. The hepatopancreatic prostaglandin E2content increased in the ARA group, and this increase was inhibited by ASA (P< 0.05). ARA decreased the lipid content in the hepatopancreas, whereas ASA recovered lipid content to a significant level (P< 0.05). ARA significantly decreased the messenger RNA (mRNA) expression levels of fatty acid synthase and stearoyl-CoA desaturase in the hepatopancreas (P< 0.05). However, ASA did not rescue the mRNA expression of these genes (P> 0.05). Interestingly, ARA significantly enhanced the level of peroxisome proliferator-activated receptor α gene expression, and this increase was attenuated by ASA (P< 0.05). Finally, ARA significantly enhanced the mRNA expression of myeloid differentiation factor 88 (MyD88) in the kidney, and ASA attenuated the expression of toll-like receptor 22 and MyD88 (P< 0.05). In conclusion, our findings suggest that COX metabolites play important roles in the inhibition of lipid accumulation in the hepatopancreas of grass carp fed with ARA and that regulation of gene expression promotes lipid catabolism rather than lipogenic activities. Additionally, these eicosanoids might participate in the upregulation of immunity-related genes in the kidney.
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影响因子：
4.5
作者：
J. Tian;H. Ji;H. Oku;Jishu Zhou
通讯作者：
J. Tian;H. Ji;H. Oku;Jishu Zhou
影响因子：
2
作者：
Atalah, Eyad;Hernandez-Cruz, Carmen Maria;Izquierdo, Marisol
通讯作者：
Izquierdo, Marisol
影响因子：
3.5
作者：
V. Kiron;J. Thawonsuwan;A. Panigrahi;J. Scharsack;S. Satoh
通讯作者：
V. Kiron;J. Thawonsuwan;A. Panigrahi;J. Scharsack;S. Satoh
影响因子：
12.4
作者：
Ricardo Weinlich;K. Bortoluci;C. F. Chehab;C. H. Serezani;A. G. Ulbrich;Marc Peters-Golden;Momtchilo Russo;G. Amarante-Mendes
通讯作者：
Ricardo Weinlich;K. Bortoluci;C. F. Chehab;C. H. Serezani;A. G. Ulbrich;Marc Peters-Golden;Momtchilo Russo;G. Amarante-Mendes
影响因子：
4.5
作者：
M. Villalta;A. Estévez;M. Bransden
通讯作者：
M. Villalta;A. Estévez;M. Bransden