Cooperative microbial interactions drive spatial segregation in porous environments.
Cooperative microbial interactions drive spatial segregation in porous environments.
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DOI：
10.1038/s41467-023-39991-4
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发表时间：
2023-07-15
影响因子：
16.6
通讯作者：
Cai, Peng
中科院分区：
文献类型：
--
作者：
Wu, Yichao;Fu, Chengxia;Peacock, Caroline L.;Sorensen, Soren J.;Redmile-Gordon, Marc A.;Xiao, Ke-Qing;Gao, Chunhui;Liu, Jun;Huang, Qiaoyun;Li, Zixue;Song, Peiyi;Zhu, Yongguan;Zhou, Jizhong;Cai, Peng
微生物相互作用的作用和形成复杂生物膜群落的潜在机制尚不清楚。本研究采用微流控芯片来表征多孔的地下环境，并表明自由生活和形成生物膜的细菌之间的合作微生物相互作用触发了主动的空间隔离，从而促进了它们各自在隔离的微栖息地中的优势地位。在最初的定植过程中，自由生活和形成生物膜的微生物从混合的浮游接种物中分离出来，占据环境流体和颗粒表面。与竞争导致的空间排斥相反，主动的空间隔离是由合作相互作用引起的，这提高了生物膜和浮游生物种群的适合度。我们进一步表明，自由生活的节杆菌通过清除生物膜抑制剂d -氨基酸诱导表面定植，并从形成生物膜的菌株分泌的公共产品中获益。总的来说，我们的研究结果揭示了合作微生物相互作用如何有助于微生物在分离的微栖息地中共存，并推动地下生物膜群落演替。在空间结构的微生物栖息地中，合作互动可能是重要的。在这里，作者表明浮游生物和生物膜形成细菌之间的合作相互作用驱动空间隔离，从而使物种在微流控芯片环境中共存。
The role of microbial interactions and the underlying mechanisms that shape complex biofilm communities are poorly understood. Here we employ a microfluidic chip to represent porous subsurface environments and show that cooperative microbial interactions between free-living and biofilm-forming bacteria trigger active spatial segregation to promote their respective dominance in segregated microhabitats. During initial colonization, free-living and biofilm-forming microbes are segregated from the mixed planktonic inoculum to occupy the ambient fluid and grain surface. Contrary to spatial exclusion through competition, the active spatial segregation is induced by cooperative interactions which improves the fitness of both biofilm and planktonic populations. We further show that free-living Arthrobacter induces the surface colonization by scavenging the biofilm inhibitor, D-amino acids and receives benefits from the public goods secreted by the biofilm-forming strains. Collectively, our results reveal how cooperative microbial interactions may contribute to microbial coexistence in segregated microhabitats and drive subsurface biofilm community succession. Cooperative interactions might be important in spatially structured microbial habitats. Here, the authors show that cooperative interactions between planktonic and biofilm-forming bacteria drive spatial segregation, and thereby enable species’ coexistence, in a microfluidic chip environment.
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影响因子：
11
作者：
Eigentler, Lukas;Kalamara, Margarita;Ball, Graeme;MacPhee, Cait E.;Stanley-Wall, Nicola R.;Davidson, Fordyce A.
通讯作者：
Davidson, Fordyce A.
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
5.2
作者：
Aliashkevich A;Alvarez L;Cava F
通讯作者：
Cava F
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
初始接种比例调节细菌共培养相互作用和代谢能力
DOI：
10.1038/s41396-020-00751-7
发表时间：
2021-01
期刊：
The ISME journal
影响因子：
--
作者：
Gao CH;Cao H;Cai P;Sørensen SJ
通讯作者：
Sørensen SJ