Oroxylum indicum (L.) Kurz extract inhibits adipogenesis and lipase activity in vitro.
Oroxylum indicum (L.) Kurz extract inhibits adipogenesis and lipase activity in vitro.
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DOI：
10.1186/s12906-018-2244-3
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发表时间：
2018-06-08
影响因子：
--
通讯作者：
Eumkeb G
中科院分区：
文献类型：
--
作者：
Hengpratom T;Lowe GM;Thumanu K;Suknasang S;Tiamyom K;Eumkeb G
籼稻（Oroxylum indicum）库尔兹（O. indicum）产于泰国。它已被用于治疗肥胖。本研究旨在探讨籼米提取物（OIE）对3T3-L1脂肪细胞成脂和生物分子变化的影响。初步研究检查了世界动物卫生组织的化学成分。3T3-L1细胞系被用来确定OIE在脂肪前细胞向脂肪细胞分化过程中的潜在毒性作用。观察OIE对3T3-L1细胞脂质积累的抑制作用。此外，还测定了OIE对胰腺脂肪酶活性的影响。在进一步的实验中，傅里叶变换红外（FTIR）用于监测和区分OIE对3T3-L1细胞潜在的抗脂肪作用所引起的生物分子变化。化学筛选结果表明，OIE主要由黄酮类、生物碱类、甾体类、糖苷类和单宁类化合物组成。与对照细胞相比，分别暴露于200或150 μg/mL的OIE 2天和10天后，3T3-L1细胞的存活率均未显著降低。与对照组相比，OIE显示出剂量依赖性的脂质积累减少（p < 0.05）。与对照组相比，提取物还显示出对脂肪酶活性的剂量依赖性抑制作用。FTIR显微光谱也证实了OIE对3T3-L1细胞脂质积累的抑制作用。oie处理的3T3-L1脂肪细胞的信号强度和脂质、脂质酯、核酸、糖原和碳水化合物相关的综合区域显著低于未处理的3T3-L1脂肪细胞（p < 0.05）。主成分分析（PCA）显示基于生物分子变化（脂质、蛋白质、核酸和碳水化合物）的3T3-L1脂肪细胞的FTIR光谱有四个不同的簇。使用无监督分层聚类分析（UHCA）证实了这一观察结果。这些新发现提供了证据，证明从植物果荚中提取的OIE能够抑制脂肪细胞中脂质和碳水化合物的积累，并且还具有抑制与脂肪吸收相关的酶的潜力。初步观察结果表明，世界动物卫生组织可能具有重要的特性，将来可用于超重或肥胖的管理。
Oroxylum indicum (L.) Kurz (O. indicum) is found in Thailand. It has been used for the treatment of obesity. This study aimed to investigate the effects of an O. indicum extract (OIE) on the adipogenic and biomolecular change in 3T3-L1 adipocytes. Initial studies examined the chemical components of OIE. The cell line 3T3-L1 was used to establish potential toxic effects of OIE during the differentiation of pre-adipocytes to adipocytes. The inhibitory effect of OIE on lipid accumulation in 3T3-L1 cells was investigated. Moreover, the impact of OIE on pancreatic lipase activity was determined. In further experiments, Fourier Transform Infrared (FTIR) was used to monitor and discriminate biomolecular changes caused by the potential anti-adipogenic effect of OIE on 3T3-L1 cells. Chemical screening methods indicated that OIE was composed of flavonoids, alkaloids, steroids, glycosides, and tannins. The percentage viability of 3T3-L1 cells was not significantly decreased after exposure to either 200 or 150 μg/mL of OIE for 2 and 10 days, respectively compared to control cells. The OIE exhibited a dose-dependent reduction of lipid accumulation compared to the control (p < 0.05). The extract also demonstrated a dose-dependent inhibitory effect upon lipase activity compared to the control. The inhibitory effect of the OIE on lipid accumulation in 3T3-L1 cells was also confirmed using FTIR microspectroscopy. The signal intensity and the integrated areas relating to lipids, lipid esters, nucleic acids, glycogen and carbohydrates of the OIE-treated 3T3-L1 adipocytes were significantly lower than the non-treated 3T3-L1 adipocytes (p < 0.05). Principal component analysis (PCA) indicated four distinct clusters for the FTIR spectra of 3T3-L1 adipocytes based on biomolecular changes (lipids, proteins, nucleic acids, and carbohydrates). This observation was confirmed using Unsupervised hierarchical cluster analysis (UHCA). These novel findings provide evidence that the OIE derived from the fruit pods of the plant is capable of inhibiting lipid and carbohydrate accumulation in adipocytes and also has the potential to inhibit an enzyme associated with fat absorption. The initial observations indicate that OIE may have important properties which in the future may be exploited for the management of the overweight or obese.
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影响因子：
5.6
作者：
Cao J;Ng ES;McNaughton D;Stanley EG;Elefanty AG;Tobin MJ;Heraud P
通讯作者：
Heraud P
影响因子：
5.6
作者：
Roh C;Jung U
通讯作者：
Jung U
影响因子：
3.7
作者：
Mandlik V;Patil S;Bopanna R;Basu S;Singh S
通讯作者：
Singh S
影响因子：
5.8
作者：
Chang, J
通讯作者：
Chang, J
影响因子：
4.3
作者：
Seo, Min-Jung;Choi, Hyeon-Son;Lee, Boo-Yong
通讯作者：
Lee, Boo-Yong