Screening of crude plant extracts with anti-obesity activity.
Screening of crude plant extracts with anti-obesity activity.
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DOI：
10.3390/ijms13021710
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发表时间：
2012
影响因子：
5.6
通讯作者：
Jung U
中科院分区：
文献类型：
--
作者：
Roh C;Jung U
肥胖是一个全球性的健康问题。众所周知，它也是代谢紊乱、2型糖尿病、全身性高血压、心血管疾病、血脂异常和动脉粥样硬化发生的危险因素。在这项研究中，我们从400种植物中筛选粗提取物，以测试其抗肥胖活性，使用猪胰脂肪酶测定（PPL;三酰甘油脂肪酶，EC 3.1.1.3）在体外活性。以2，4-二硝基苯丁酸酯为底物，在400种植物中，有44种植物提取物表现出较高的抗脂肪酶活性。此外，研究了44种植物提取物对3 T3-L1细胞脂质积累的抑制作用。在这44种提取物中，有4种天然植物的粗提物具有活性。在3 T3-L1脂肪细胞中，丹参皮具有最高的脂肪抑制活性，而鲁比、山茱萸和大老姜在100 μg/mL下显示出高于30%的脂肪抑制能力，表明具有抗肥胖活性。这些结果表明，四种有效的植物提取物可能对治疗肥胖症具有治疗意义。
Obesity is a global health problem. It is also known to be a risk factor for the development of metabolic disorders, type 2 diabetes, systemic hypertension, cardiovascular disease, dyslipidemia, and atherosclerosis. In this study, we screened crude extracts from 400 plants to test their anti-obesity activity using porcine pancreatic lipase assay (PPL; triacylglycerol lipase, EC 3.1.1.3) in vitro activity. Among the 400 plants species examined, 44 extracts from plants, showed high anti-lipase activity using 2,4-dinitrophenylbutyrate as a substrate in porcine pancreatic lipase assay. Furthermore, 44 plant extracts were investigated for their inhibition of lipid accumulation in 3T3-L1 cells. Among these 44 extracts examined, crude extracts from 4 natural plant species were active. Salicis Radicis Cortex had the highest fat inhibitory activity, whereas Rubi Fructus, Corni Fructus, and Geranium nepalense exhibited fat inhibitory capacity higher than 30% at 100 μg/mL in 3T3-L1 adipocytes, suggesting anti-obesity activity. These results suggest that four potent plant extracts might be of therapeutic interest with respect to the treatment of obesity.
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