Visualising nanoscale restructuring of a cellular membrane triggered by polyelectrolyte microcapsules.
Visualising nanoscale restructuring of a cellular membrane triggered by polyelectrolyte microcapsules.
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DOI：
10.1039/c8nr03870h
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发表时间：
2018-09-13
期刊：
影响因子：
6.7
通讯作者：
Novak P
中科院分区：
文献类型：
--
作者：
Chen Y ;Sukhorukov GB ;Novak P
微胶囊内化的地形和弹性模量的同时延时成像揭示了膜突起的不同纳米级重构。基于聚合物的多层微囊化技术代表了细胞内药物递送的有前途的策略之一，然而，涉及载体内化的膜过程尚未完全理解。在这里，我们采用了扫描探针显微镜技术，称为扫描离子电导显微镜（SICM），研究这些复杂的过程在纳米级的分辨率在真实的时间。我们能够在A549细胞培养物中的微胶囊内化的整个过程中同时成像形貌和局部弹性模量，而不破坏内化过程。成像显示，胶囊触发了其附近膜突起的形成，这是实现完全胶囊内化的重要但不充分的一步。一个关键的方面似乎是纳米级重组这些突起到光滑的薄层延伸到胶囊的表面。胶囊内化过程中的弹性模量的同时映射允许监测膜片材在胶囊表面上的延伸过程中的结构变化以及随后的胶囊屈曲的内化后现象。据我们所知，这些是第一个实验数据捕捉细胞膜和微胶囊之间的相互作用，在其整个复杂性与纳米级分辨率。这里建立的方法有可能提供新的见解之间的界面的纳米结构材料和细胞膜在生理条件下的相互作用。
Simultaneous time-lapse imaging of topography and elastic modulus of microcapsule internalisation reveals distinct nanoscale restructuring of membrane protrusions. Polymer-based multilayer microencapsulation technology represents one of the promising strategies for intracellular drug delivery, however, membrane processes involved in vehicle internalisation are not fully understood. Here we employed a scanning probe microscopy technique called Scanning Ion Conductance Microscopy (SICM) to study these complex processes at nanoscale resolution in real time. We were able to image topography simultaneously with local elastic modulus throughout the whole course of microcapsule internalisation in A549 cell culture without disrupting the internalisation process. The imaging revealed that capsules triggered the formation of membrane protrusions in their vicinity, which is an important but not a sufficient step towards full capsule internalisation. A crucial aspect appeared to be nanoscale restructuring of these protrusions into smooth thin layers extending over the surface of capsules. Simultaneous mapping of elastic modulus during capsule internalisation allowed monitoring the structural changes during extension of the membrane sheets over the surface of the capsule and the subsequent post-internalisation phenomenon of capsule buckling. To our knowledge these are the first experimental data capturing the interactions between the cellular membrane and microcapsules in their whole complexity with nanoscale resolution. The methodology established here has the potential to provide new insights into interactions at the interface between the nanostructured materials and cellular membrane under physiological conditions.
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DOI：
10.1083/jcb.201109130
发表时间：
2012-05-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Shevchuk AI;Novak P;Taylor M;Diakonov IA;Ziyadeh-Isleem A;Bitoun M;Guicheney P;Lab MJ;Gorelik J;Merrifield CJ;Klenerman D;Korchev YE
通讯作者：
Korchev YE
影响因子：
48
作者：
Novak, Pavel;Li, Chao;Shevchuk, Andrew I.;Stepanyan, Ruben;Caldwell, Matthew;Hughes, Simon;Smart, Trevor G.;Gorelik, Julia;Ostanin, Victor P.;Lab, Max J.;Moss, Guy W. J.;Frolenkov, Gregory I.;Klenerman, David;Korchev, Yuri E.
通讯作者：
Korchev, Yuri E.
影响因子：
17.1
作者：
Kastl, Lena;Sasse, Daniel;Rivera Gil, Pilar
通讯作者：
Rivera Gil, Pilar
影响因子：
10.8
作者：
Novak, Pavel;Shevchuk, Andrew;Korchev, Yuri E.
通讯作者：
Korchev, Yuri E.
DOI：
10.1038/nrm2447
发表时间：
2008-08
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
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