Identification, characterization, and transcription analysis of xylogen-like arabinogalactan proteins in rice (Oryza sativa L.).
Identification, characterization, and transcription analysis of xylogen-like arabinogalactan proteins in rice (Oryza sativa L.).
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水稻 (Oryza sativa L.) 中类木质素阿拉伯半乳聚糖蛋白的鉴定、表征和转录分析。
DOI：
10.1186/s12870-014-0299-y
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发表时间：
2014-11-18
影响因子：
5.3
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Ma T;Ma H;Zhao H;Qi H;Zhao J
木质素是一种含有非特异性脂质转移蛋白结构域的阿拉伯半乳聚糖嵌合蛋白，可促进木质部细胞分化。目前尚未对水稻XYLP基因家族进行全面的研究。因此，作为研究该基因家族的第一步，并作为一般有用的策略，需要对OsXYLP基因家族进行全基因组分析。本研究从水稻基因组中鉴定了21个XYLP基因，并对它们的蛋白质结构、系统发育关系、染色体定位和基因复制状态进行了全面分析。我们的研究结果表明，基因重复在OsXYLP基因家族的扩展中发挥了重要作用。我们使用表达序列标签，芯片，大规模平行签名测序，和定量实时PCR数据分析OsXYLP基因在不同发育阶段和非生物胁迫条件下的表达。我们发现许多OsXYLP基因在维管组织和种子中大量表达，其中一些基因受激素或非生物胁迫的调控。此外，我们还鉴定了OsXYLP 7和OsXYLP 16的敲除突变体，发现突变体xylp 7在茎高方面存在缺陷。我们分析了21个XYLP基因的表达谱，并表征了其蛋白质的结构和进化关系。我们的研究结果表明，水稻XYLP基因家族可能在植物维管系统发育和激素信号转导中发挥作用。在检测到的21个OsXYLPs中，19个是新鉴定的编码阿拉伯半乳聚糖蛋白的基因。我们的研究结果提供了全面的见解，将有助于未来的研究水稻XYLP基因家族的生物学功能。本文的在线版本（doi：10.1186/s12870-014-0299-y）包含补充材料，可供授权用户使用。
Xylogen, a chimeric arabinogalactan protein containing a non-specific lipid transfer protein domain, can promote xylem cell differentiation. No comprehensive study has been carried out on the XYLP gene family in rice. As a first step in research on this gene family and as a useful strategy in general, a genome-wide analysis of the OsXYLP gene family is thus needed. In this study, we identified 21 XYLP genes from the rice genome and comprehensively analyzed their protein structures, phylogenetic relationships, chromosomal locations, and gene duplication status. Our results indicate that gene duplication has played major roles in the expansion of the OsXYLP gene family. We used expressed sequence tag, microarray, massively parallel signature sequencing, and quantitative real-time PCR data to analyze OsXYLP gene expression during various developmental stages and under abiotic stress conditions. We found that many OsXYLP genes are abundantly expressed in vascular tissues and seeds, with some genes regulated under hormonal or abiotic stresses. In addition, we identified knockout mutants of OsXYLP7 and OsXYLP16 and discovered that the mutant xylp7 has a defect in stem height. We analyzed expression profiles of 21 XYLP genes and characterized the structures and evolutionary relationships of their proteins. Our results demonstrate that the rice XYLP gene family may play roles in plant vascular system development and hormone signaling. Among the 21 detected OsXYLPs, 19 are newly identified genes encoding arabinogalactan proteins. Our results provide comprehensive insights that will assist future research on the biological functions of the rice XYLP gene family. The online version of this article (doi:10.1186/s12870-014-0299-y) contains supplementary material, which is available to authorized users.
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水稻阿拉伯半乳聚糖蛋白基因家族的全基因组鉴定、分类和表达分析（Oryza sativa L.）
影响因子：
6.9
作者：
Ma H;Zhao J
通讯作者：
Zhao J
DOI：
10.1073/pnas.96.18.10284
发表时间：
1999-08-31
影响因子：
11.1
作者：
Ashikari, M;Wu, JZ;Yoshimura, A
通讯作者：
Yoshimura, A
影响因子：
4.9
作者：
Mashiguchi, K;Yamaguchi, I;Suzuki, Y
通讯作者：
Suzuki, Y
影响因子：
7.2
作者：
Levitin, Bella;Richter, Dganit;Zik, Moriyah
通讯作者：
Zik, Moriyah
影响因子：
7.4
作者：
Jain, Mukesh;Nijhawan, Aashima;Khurana, Jitendra P.
通讯作者：
Khurana, Jitendra P.