Molecular and functional characterization of the luqin-type neuropeptide signaling system in the sea cucumber Apostichopus japonicus
Molecular and functional characterization of the luqin-type neuropeptide signaling system in the sea cucumber Apostichopus japonicus
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海参芦荻型神经肽信号系统的分子和功能表征
DOI：
10.1016/j.peptides.2022.170839
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发表时间：
2022-07
期刊：
影响因子：
--
通讯作者：
Muyan Chen
中科院分区：
文献类型：
--
作者：
Chenyi Li;Yingqiu Zheng;Xiao Cong;Huachen Liu;Kenneth B Storey;Muyan Chen
海洋无脊椎动物神经肽的功能特性近年来引起了人们的广泛关注，但对陆琴类神经肽的功能研究还很有限，尤其是在后口动物中。仿刺参（Apostichopus）是后口门海参纲无脊椎动物的代表物种。该物种在中国具有很高的营养价值和药用价值。在这项研究中，我们报告了第一个全面的组织学，生化和药理学特征的luqin型神经肽信号在海参A。- 是的助理鲁秦样神经肽前体（AjLQP）含有一个典型的后口动物鲁秦样神经肽AjLQ，具有xFxRW酰胺基序。AjLQ被鉴定为luqin型神经肽受体AjLQR的配体，该受体先前被预测为速激肽型受体，并触发细胞内Ca 2+的快速动员，随后是受体内化和ERK 1/2磷酸化的瞬时增加。原位杂交、免疫组化和qRT-PCR分析显示AjLQP和AjLQ在A.运动组织，尤其是与运动有关的器官。离体药理实验表明，AjLQ在10 - 7 M浓度下对纵行肌的舒张作用为12.69% ± 1.99%（P <0.01）。另外，我们观察到AjLQP在深夏海参肠中的表达显著增加（约2.37倍，p <0.01），这表明AjLQ可能参与了深夏海参的摄食抑制。本研究提供了第一次深入了解海参luqin型神经肽信号的实验特征。该结果将拓宽我们对神经肽在海洋无脊椎动物重要生物过程中潜在功能的认识，并为优化海参养殖技术提供理论支持。·Luqin型神经肽AjLQ及其前体AjLQP具有广泛的表达模式，特别是在运动相关器官中。·Luqin型神经肽受体AjLQR一旦被AjLQ激活，可激活ERK 1/2级联。· AjLQ引起A的弛豫。纵肌。· AjLQ可能与A. - 是的
The functional characteristics of neuropeptides in marine invertebrates have attracted significant attention recently although functional studies of luqin-type neuropeptides are still very limited, especially in deuterostomes. The sea cucumber, Apostichopus japonicus , is a representative species of deuterostomian Holothurian invertebrates. The species has high nutritional and medicinal value in China. In this study, we report the first comprehensive histological, biochemical and pharmacological characterization of luqin-type neuropeptide signaling in the sea cucumber A. japonicus . The A. japonicus luqin-like neuropeptide precursor (AjLQP) contains a single typical deuterostomian luqin-like neuropeptide AjLQ with an xFxRWamide motif. AjLQ was identified as the ligand for a luqin-type neuropeptide receptor AjLQR, that was previously predicted to be a tachykinin-type receptor, and triggers a rapid intracellular mobilization of Ca 2+ , followed by receptor internalization and a transient increase in ERK1/2 phosphorylation. In situ hybridization, immunohistochemistry and qRT-PCR analysis revealed extensive expression of AjLQP and AjLQ in A. japonicus tissues, especially in locomotion-related organs. In vitro pharmacological tests revealed that AjLQ caused 12.69% ± 1.99% ( p <0.01) relaxation of longitudinal muscle preparations at 10 -7 M concentration. Furthermore, we observed significantly increased expression of AjLQP (about 2.37 fold, p <0.01) in intestine of deeply aestivating sea cucumbers, which suggests that AjLQ might be involved in feeding inhibition during aestivation. The present study provides a first insight into the experimental characterization of luqin-type neuropeptide signaling in a sea cucumber. The results will broaden our understanding of the potential function of neuropeptides during important biological processes in marine invertebrates and provide theoretical support for optimizing sea cucumber aquaculture technology. • Luqin-type neuropeptide AjLQ and its precursor AjLQP have an extensive expression pattern especially in locomotion-related organs. • Luqin-type neuropeptide receptor AjLQR once activated by AjLQ can activate ERK1/2 cascade. • AjLQ causes relaxation of A. japonicus longitudinal muscle. • AjLQ might be involved in feeding behavior and aestivation in A. japonicus .
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影响因子：
4.6
作者：
Nguyen TV;Cummins SF;Elizur A;Ventura T
通讯作者：
Ventura T
影响因子：
3.7
作者：
Schwerdtner Máñez K;Ferse SC
通讯作者：
Ferse SC
影响因子：
5.2
作者：
Kawada T;Shiraishi A;Matsubara S;Hozumi A;Horie T;Sasakura Y;Satake H
通讯作者：
Satake H
DOI：
--
发表时间：
2015
期刊：
--
影响因子：
--
作者：
Hongsheng Yang;J. Hamel;A. Mercier
通讯作者：
Hongsheng Yang;J. Hamel;A. Mercier
DOI：
10.1073/pnas.1910262116
发表时间：
2020-01-14
影响因子：
11.1
作者：
Andreatta，Gabriele;Broyart，Caroline;Raible，Florian
通讯作者：
Raible，Florian