Drosophila melanogaster as a model host for the Burkholderia cepacia complex.
Drosophila melanogaster as a model host for the Burkholderia cepacia complex.
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DOI：
10.1371/journal.pone.0011467
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发表时间：
2010-07-12
期刊：
影响因子：
3.7
通讯作者：
Déziel E
中科院分区：
文献类型：
--
作者：
Castonguay-Vanier J;Vial L;Tremblay J;Déziel E
洋葱伯克霍尔德菌复合体 (Bcc) 细菌的定植与囊性纤维化患者的健康状况快速下降有关。为了研究 Bcc 的毒力，已经开发了几种替代感染模型。为此，果蝇越来越多地被用作替代宿主，其在增强我们对宿主与病原体关系的理解方面的有效性已通过多种微生物得到证明。此外，它作为哺乳动物宿主的合适替代品的相关性已在脊椎动物中得到证实。本研究的目的是建立黑腹果蝇作为 Bcc 物种的替代宿主。虽然喂食方法并不能成功杀死苍蝇，但刺刺技术确实导致了苍蝇种群的死亡。使用果蝇模型获得的结果与使用哺乳动物感染模型获得的结果相当。此外，果蝇感染模型的有效性得到了新洋葱伯克霍尔德杆菌 K56-2 突变体的证实，已知该突变体在小鼠宿主或其他替代模型中毒性较低。还使用果蝇作为宿主进行了竞争指数（CI）分析。 CI 实验的结果与哺乳动物模型获得的结果一致。我们得出的结论是，果蝇是 Bcc 的一种有用的替代感染模型，并且蝇刺测定和竞争指数是毒力测试的两种补充方法。此外，CI 结果表明该方法比死亡率测试更敏感。
Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis. In order to investigate the virulence of the Bcc, several alternative infection models have been developed. To this end, the fruit fly is increasingly used as surrogate host, and its validity to enhance our understanding of host-pathogen relationships has been demonstrated with a variety of microorganisms. Moreover, its relevance as a suitable alternative to mammalian hosts has been confirmed with vertebrate organisms. The aim of this study was to establish Drosophila melanogaster as a surrogate host for species from the Bcc. While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations. Results obtained with the fruit fly model are comparable with results obtained using mammalian infection models. Furthermore, validity of the Drosophila infection model was confirmed with B. cenocepacia K56-2 mutants known to be less virulent in murine hosts or in other alternative models. Competitive index (CI) analyses were also performed using the fruit fly as host. Results of CI experiments agree with those obtained with mammalian models. We conclude that Drosophila is a useful alternative infection model for Bcc and that fly pricking assays and competition indices are two complementary methods for virulence testing. Moreover, CI results indicate that this method is more sensitive than mortality tests.
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DOI：
10.1099/00207713-51-4-1481
发表时间：
2001-07-01
影响因子：
2.8
作者：
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