A High-Resolution Minimicroscope System for Wireless Real-Time Monitoring
A High-Resolution Minimicroscope System for Wireless Real-Time Monitoring
复制标题
用于无线实时监测的高分辨率微型显微镜系统
DOI：
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发表时间：
2018
影响因子：
4.6
通讯作者：
Keekyoung Kim
中科院分区：
文献类型：
--
作者：
Zongjie Wang;Akash Boddeda;Benjamin Parker;R. Samanipour;Sanjoy Ghosh;F. Ménard;Keekyoung Kim
目的：细胞生物学和生物医学工程对紧凑、高性价比、高性能显微镜的要求很高，它可以对细胞和芯片实验室设备进行实时成像。介绍了一种低成本无线微型显微镜的设计和应用，其分辨率可达2592 × 1944像素，速度可达90 f/s。方法：微型显微镜系统构建在商业嵌入式系统（Raspberry Pi）上。我们改进了一个摄像模块，采用了一个倒置的双透镜系统，以获得清晰的视野和适当的放大倍数为几十微米的目标。结果：该系统能够采集延时图像并无线传输图像数据。整个系统可以在传统的细胞培养箱中无线和无绳操作。利用所研制的微型显微镜对NIH-3 T3和HEK 293细胞在培养箱中50 h的贴壁和增殖进行了监测。此外，微型显微镜用于监测微流体装置中的液滴生成过程。微型显微镜捕获的高质量图像使我们能够自动分析实验参数。结论：成功的应用证明了所开发的微型显微镜在监测各种生物样品和微流体装置方面的巨大潜力。重要性：本文介绍了一种高分辨率微型显微镜系统的设计，使无线实时成像的细胞培养箱内。该系统已被证实是一个有用的工具，以获得高质量的图像和视频的自动定量分析的生物样品和芯片实验室设备在长期的。
Objective: Compact, cost-effective, and high-performance microscope that enables the real-time imaging of cells and lab-on-a-chip devices is highly demanded for cell biology and biomedical engineering. This paper aims to present the design and application of an inexpensive wireless minimicroscope with resolution up to 2592 × 1944 pixels and speed up to 90 f/s. Methods: The minimicroscope system was built on a commercial embedded system (Raspberry Pi). We modified a camera module and adopted an inverse dual lens system to obtain the clear field of view and appropriate magnification for tens of micrometer objects. Results: The system was capable of capturing time-lapse images and transferring image data wirelessly. The entire system can be operated wirelessly and cordlessly in a conventional cell culturing incubator. The developed minimicroscope was used to monitor the attachment and proliferation of NIH-3T3 and HEK 293 cells inside an incubator for 50 h. In addition, the minimicroscope was used to monitor a droplet generation process in a microfluidic device. The high-quality images captured by the minimicroscope enabled us an automated analysis of experimental parameters. Conclusion: The successful applications prove the great potential of the developed minimicroscope for monitoring various biological samples and microfluidic devices. Significance: This paper presents the design of a high-resolution minimicroscope system that enables the wireless real-time imaging of cells inside the incubator. This system has been verified to be a useful tool to obtain high-quality images and videos for the automated quantitative analysis of biological samples and lab-on-a-chip devices in the long term.
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影响因子：
7.4
作者：
Seo, Sungkyu;Isikman, Serhan O.;Sencan, Ikbal;Mudanyali, Onur;Su, Ting-Wei;Bishara, Waheb;Erlinger, Anthony;Ozcan, Aydogan
通讯作者：
Ozcan, Aydogan
用于同步微流控精子监测和分选的无透镜成像。
影响因子：
6.1
作者：
Zhang,Xiaohui;Khimji,Imran;Gurkan,UmutAtakan;Safaee,Hooman;Catalano,PaoloNicolas;Keles,HasanOnur;Kayaalp,Emre;Demirci,Utkan
通讯作者：
Demirci,Utkan
影响因子：
6.1
作者：
Tseng D;Mudanyali O;Oztoprak C;Isikman SO;Sencan I;Yaglidere O;Ozcan A
通讯作者：
Ozcan A
影响因子：
6.1
作者：
Zhang YS;Ribas J;Nadhman A;Aleman J;Selimović Š;Lesher-Perez SC;Wang T;Manoharan V;Shin SR;Damilano A;Annabi N;Dokmeci MR;Takayama S;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
6.1
作者：
Kim SB;Bae H;Cha JM;Moon SJ;Dokmeci MR;Cropek DM;Khademhosseini A
通讯作者：
Khademhosseini A