Development of a SERS-based lateral flow immunoassay for rapid and ultra-sensitive detection of anti-SARS-CoV-2 IgM/IgG in clinical samples.
Development of a SERS-based lateral flow immunoassay for rapid and ultra-sensitive detection of anti-SARS-CoV-2 IgM/IgG in clinical samples.
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DOI：
10.1016/j.snb.2020.129196
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发表时间：
2021-02-15
期刊：
影响因子：
--
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Liu H;Dai E;Xiao R;Zhou Z;Zhang M;Bai Z;Shao Y;Qi K;Tu J;Wang C;Wang S
准确、快速地筛查抗SARS冠状病毒2型血清抗体是控制2019年冠状病毒病(新冠肺炎)传播的关键。在这项研究中，我们报道了一种基于表面增强拉曼散射的侧向流动免疫分析(SERS-LFIA)，用于同时检测抗SARS-CoV-2IgM/Ig G，并具有高灵敏度。通过在SiO_2核(SiO_2@Ag)表面涂覆一层完整的银壳，制备了双层拉曼染料标记的新型SERS标签，该标签具有良好的SERS信号、良好的单分散性和高的稳定性。将抗人Ig M和Ig G固定在试条的两条测试线上，捕获形成的SiO_2@Ag-Spike(S)蛋白-抗SARS-CoV-2Ig M/Ig G免疫复合物。用便携式拉曼仪可以方便地记录IgM和IgG测试区的SERS信号强度，并用于目标IgM和IgG的高灵敏度分析。SERS-LFIA的检出限是标准的金纳米颗粒LFIA的800倍。通过对19份新冠肺炎患者阳性血清和49份健康人阴性血清样本的检测，验证了该传感器的临床可行性。结果表明，该方法对SARS-CoV-2感染患者具有较高的准确性和特异性。
The accurate and rapid screening of serum antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the key to control the spread of 2019 coronavirus disease (COVID-19). In this study, we reported a surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA) for the simultaneous detection of anti-SARS-CoV-2 IgM/IgG with high sensitivity. Novel SERS tags labeled with dual layers of Raman dye were fabricated by coating a complete Ag shell on SiO2 core (SiO2@Ag) and exhibited excellent SERS signals, good monodispersity, and high stability. Anti-human IgM and IgG were immobilized onto the two test lines of the strip to capture the formed SiO2@Ag-spike (S) protein-anti-SARS-CoV-2 IgM/IgG immunocomplexes. The SERS signal intensities of the IgM and IgG test zones were easily recorded by a portable Raman instrument and used for the high-sensitivity analysis of target IgM and IgG. The limit of detection of SERS-LFIA was 800 times higher than that of standard Au nanoparticle-based LFIA for target IgM and IgG. The SERS-LFIA biosensor was tested on 19 positive serum samples from COVID-19 patients and 49 negative serum samples from healthy people to demonstrate the clinical feasibility of our proposed assay. The results revealed that the proposed method exhibited high accuracy and specificity for patients with SARS-CoV-2 infection.
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