Identification of immunity-related genes in Plutella xylostella in response to fungal peptide destruxin A: RNA-Seq and DGE analysis.
Identification of immunity-related genes in Plutella xylostella in response to fungal peptide destruxin A: RNA-Seq and DGE analysis.
复制标题
鉴定小菜蛾中真菌肽 destruxin A 免疫相关基因：RNA-Seq 和 DGE 分析
DOI：
10.1038/s41598-017-11298-7
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发表时间：
2017-09-08
影响因子：
4.6
通讯作者：
Jin F
中科院分区：
文献类型：
--
作者：
Shakeel M;Xu X;Xu J;Zhu X;Li S;Zhou X;Yu J;Xu X;Hu Q;Yu X;Jin F
小菜蛾（Plutella xylostella）对多种杀虫剂具有很强的抗药性，已成为我国主要的鳞翅目害虫。绿僵菌毒素A是一种广谱的杀虫真菌。然而，破坏素A与P.在基因组水平上的木质素仍然没有很好的理解。在这里，我们确定了129个免疫相关基因，包括模式识别受体，信号调节器，主要免疫途径（Toll，Imd和JAK/STAT）的几个成员，以及免疫效应子。在三个不同的时间过程（2 h、4 h和6 h）下，xylostellain对destruxin A的响应。值得一提的是，免疫相关差异表达基因（DEG）分析显示，在P中有30，78和72个上调基因和17，13和6个下调基因。与对照相比，在注射破坏菌素A后2 h、4 h和6 h，有趣的是，我们的研究结果显示，在昆虫免疫系统中起重要作用的抗菌肽的表达在注射destruxin A后上调。我们的研究结果提供了免疫相关DEG的详细信息，并揭示了P的潜力。xylostellata通过增加抗菌肽的活性来限制真菌肽destruxin A的感染。
Plutella xylostellahas become the major lepidopteran pest ofBrassicaowing to its strong ability of resistance development to a wide range of insecticides. Destruxin A, a mycotoxin of entomopathogenic fungus,Metarhizium anisopliae, has broad-spectrum insecticidal effects. However, the interaction mechanism of destruxin A with the immune system ofP. xylostellaat genomic level is still not well understood. Here, we identified 129 immunity-related genes, including pattern recognition receptors, signal modulators, few members of main immune pathways (Toll, Imd, and JAK/STAT), and immune effectors inP. xylostellain response to destruxin A at three different time courses (2 h, 4 h, and 6 h). It is worthy to mention that the immunity-related differentially expressed genes (DEGs) analysis exhibited 30, 78, and 72 up-regulated and 17, 13, and 6 down-regulated genes inP. xylostellaafter destruxin A injection at 2 h, 4 h, and 6 h, respectively, compared to control. Interestingly, our results revealed that the expression of antimicrobial peptides that play a vital role in insect immune system was up-regulated after the injection of destruxin A. Our findings provide a detailed information on immunity-related DEGs and reveal the potential ofP. xylostellato limit the infection of fungal peptide destruxin A by increasing the activity of antimicrobial peptides.
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影响因子：
4.5
作者：
Gao Q;Jin K;Ying SH;Zhang Y;Xiao G;Shang Y;Duan Z;Hu X;Xie XQ;Zhou G;Peng G;Luo Z;Huang W;Wang B;Fang W;Wang S;Zhong Y;Ma LJ;St Leger RJ;Zhao GP;Pei Y;Feng MG;Xia Y;Wang C
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Wang C
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2.8
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Musa, Peter D.
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56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC
影响因子：
3.4
作者：
Bandani, AR;Amiri, B;Gordon-Weeks, R
通讯作者：
Gordon-Weeks, R
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影响因子：
4.4
作者：
Dissanayake, Sumudu N.;Marinotti, Osvaldo;Ribeiro, Jose Marcos C.;James, Anthony A.
通讯作者：
James, Anthony A.