Small-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation.
Small-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation.
复制标题
DOI：
10.3390/cells10051166
复制
发表时间：
2021-05-11
期刊：
影响因子：
6
通讯作者：
Vasilaki A
中科院分区：
文献类型：
--
作者：
Kanakis I;Alameddine M;Folkes L;Moxon S;Myrtziou I;Ozanne SE;Peffers MJ;Goljanek-Whysall K;Vasilaki A
孕期和哺乳期母亲的饮食会影响后代骨骼肌的发育，并决定后代的肌肉健康状况。在本文中，我们描述了母体低蛋白饮食诱导的后代骨骼肌变化与小非编码rna （sncRNAs）差异表达（DE）之间的关系。我们使用了母体蛋白限制小鼠模型，在妊娠期间给母鼠喂食正常（N, 20%）或低蛋白（L, 8%）的饲料，并将新生儿交叉培养到N或L哺乳母鼠中，从而产生NN， NL和LN后代组。断奶NL雄性后代的全身和胫骨前肌（TA）重量下降，但LN组与NN组相比没有差异。然而，对TA肌的组织学评估显示，两组在断奶时肌纤维大小均有所减少。小rna测序显示了多个mir、snoRNAs和snrna的DE。结合已知的myomir，对miRs-15a、-34a、-122和-199a的生物信息学分析证实了它们在关键的肌肉特异性生物过程中的作用。这是关于sncrna在骨骼肌发育的营养相关编程中的DE的第一份综合报告，强调了进一步研究以揭示详细的分子机制的必要性。
Maternal diet during gestation and lactation affects the development of skeletal muscles in offspring and determines muscle health in later life. In this paper, we describe the association between maternal low protein diet-induced changes in offspring skeletal muscle and the differential expression (DE) of small non-coding RNAs (sncRNAs). We used a mouse model of maternal protein restriction, where dams were fed either a normal (N, 20%) or a low protein (L, 8%) diet during gestation and newborns were cross-fostered to N or L lactating dams, resulting in the generation of NN, NL and LN offspring groups. Total body and tibialis anterior (TA) weights were decreased in weanling NL male offspring but were not different in the LN group, as compared to NN. However, histological evaluation of TA muscle revealed reduced muscle fibre size in both groups at weaning. Small RNA-sequencing demonstrated DE of multiple miRs, snoRNAs and snRNAs. Bioinformatic analyses of miRs-15a, -34a, -122 and -199a, in combination with known myomiRs, confirmed their implication in key muscle-specific biological processes. This is the first comprehensive report for the DE of sncRNAs in nutrition-associated programming of skeletal muscle development, highlighting the need for further research to unravel the detailed molecular mechanisms.
登录
查看更多内容
DOI：
10.1002/bies.201600264
发表时间：
2017-06
期刊：
BioEssays : news and reviews in molecular, cellular and developmental biology
影响因子：
--
作者：
Falaleeva M;Welden JR;Duncan MJ;Stamm S
通讯作者：
Stamm S
影响因子：
2.9
作者：
Butchart, Lauren C.;Fox, Archa;Grounds, Miranda D.
通讯作者：
Grounds, Miranda D.
影响因子：
3.4
作者：
Confortim, Heloisa Deola;Jeronimo, Leslie Cazetta;Torrejais, Marcia Miranda
通讯作者：
Torrejais, Marcia Miranda
DOI：
10.1093/bioinformatics/btp101
发表时间：
2009-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Bindea G;Mlecnik B;Hackl H;Charoentong P;Tosolini M;Kirilovsky A;Fridman WH;Pagès F;Trajanoski Z;Galon J
通讯作者：
Galon J
DOI：
10.1083/jcb.200911036
发表时间：
2010-09-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chen JF;Tao Y;Li J;Deng Z;Yan Z;Xiao X;Wang DZ
通讯作者：
Wang DZ