Molecular cloning of doublesex genes of four cladocera (water flea) species.
Molecular cloning of doublesex genes of four cladocera (water flea) species.
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DOI：
10.1186/1471-2164-14-239
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发表时间：
2013-04-10
期刊：
影响因子：
4.4
通讯作者：
Iguchi T
中科院分区：
文献类型：
--
作者：
Toyota K;Kato Y;Sato M;Sugiura N;Miyagawa S;Miyakawa H;Watanabe H;Oda S;Ogino Y;Hiruta C;Mizutani T;Tatarazako N;Paland S;Jackson C;Colbourne JK;Iguchi T
doublex基因（dsx）在许多生物中被认为是调节遗传性别决定的关键因子。我们以前确定了两个dsx基因（DapmaDsx 1和DapmaDsx 2）从淡水鳃足类甲壳动物，大型蚤，这是在男性中表达，但不是在女性。D. magna通过响应环境线索（环境性别决定）的孤雌生殖产生雄性，并且我们表明DapmaDsx 1在胚胎阶段的表达负责雄性性状的发育。地方magna dsx基因被认为是由枝角类特异性复制产生的;因此，为了研究dsx基因性别特异性表达的进化保守性，并进一步评估它们在环境性别决定中的功能，我们在四个密切相关的枝角类物种中寻找dsx同源物。我们从D. pulex、尖尾拟步行虫D. galeata和Ceriodaphnia dubia的dsx基因，而在多刺裸腹蚤中只发现了一个dsx基因。所有9个dsx同源物的推导的氨基酸序列包含DM和寡聚化结构域，这是所有节肢动物DSX家族成员的特征。分子系统发育分析表明，dsx基因的重复可能发生在这些枝角类物种的分歧之前，因为巨型虎虾斑节对虾的起源都DSX 1和DSX 2的枝角类。因此，这一结果也表明M.其次是dsx 2基因的缺失。此外，本研究中鉴定的所有dsx基因都显示出雄性偏好的表达水平，但只有一半的5'上游调控元件在D. magna和D.蚤。5种枝角类的dsx基因具有相似的氨基酸结构，含有高度保守的DM和寡聚化结构域，并表现出性别二态性表达模式，表明这些基因在枝角类的环境性别决定中可能具有相似的功能。
The gene doublesex (dsx) is known as a key factor regulating genetic sex determination in many organisms. We previously identified two dsx genes (DapmaDsx1 and DapmaDsx2) from a freshwater branchiopod crustacean, Daphnia magna, which are expressed in males but not in females. D. magna produces males by parthenogenesis in response to environmental cues (environmental sex determination) and we showed that DapmaDsx1 expression during embryonic stages is responsible for the male trait development. The D. magna dsx genes are thought to have arisen by a cladoceran-specific duplication; therefore, to investigate evolutionary conservation of sex specific expression of dsx genes and to further assess their functions in the environmental sex determination, we searched for dsx homologs in four closely related cladoceran species. We identified homologs of both dsx genes from, D. pulex, D. galeata, and Ceriodaphnia dubia, yet only a single dsx gene was found from Moina macrocopa. The deduced amino acid sequences of all 9 dsx homologs contained the DM and oligomerization domains, which are characteristic for all arthropod DSX family members. Molecular phylogenetic analysis suggested that the dsx gene duplication likely occurred prior to the divergence of these cladoceran species, because that of the giant tiger prawn Penaeus monodon is rooted ancestrally to both DSX1 and DSX2 of cladocerans. Therefore, this result also suggested that M. macrocopa lost dsx2 gene secondarily. Furthermore, all dsx genes identified in this study showed male-biased expression levels, yet only half of the putative 5’ upstream regulatory elements are preserved in D. magna and D. pulex. The all dsx genes of five cladoceran species examined had similar amino acid structure containing highly conserved DM and oligomerization domains, and exhibited sexually dimorphic expression patterns, suggesting that these genes may have similar functions for environmental sex determination in cladocerans.
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影响因子：
64.5
作者：
BURTIS, KC;BAKER, BS
通讯作者：
BAKER, BS
DOI：
10.1126/science.1197761
发表时间：
2011-02-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colbourne JK;Pfrender ME;Gilbert D;Thomas WK;Tucker A;Oakley TH;Tokishita S;Aerts A;Arnold GJ;Basu MK;Bauer DJ;Cáceres CE;Carmel L;Casola C;Choi JH;Detter JC;Dong Q;Dusheyko S;Eads BD;Fröhlich T;Geiler-Samerotte KA;Gerlach D;Hatcher P;Jogdeo S;Krijgsveld J;Kriventseva EV;Kültz D;Laforsch C;Lindquist E;Lopez J;Manak JR;Muller J;Pangilinan J;Patwardhan RP;Pitluck S;Pritham EJ;Rechtsteiner A;Rho M;Rogozin IB;Sakarya O;Salamov A;Schaack S;Shapiro H;Shiga Y;Skalitzky C;Smith Z;Souvorov A;Sung W;Tang Z;Tsuchiya D;Tu H;Vos H;Wang M;Wolf YI;Yamagata H;Yamada T;Ye Y;Shaw JR;Andrews J;Crease TJ;Tang H;Lucas SM;Robertson HM;Bork P;Koonin EV;Zdobnov EM;Grigoriev IV;Lynch M;Boore JL
通讯作者：
Boore JL
影响因子：
11.4
作者：
Kopp, Artyom
通讯作者：
Kopp, Artyom
影响因子：
3.3
作者：
BULMER, MG;BULL, JJ
通讯作者：
BULL, JJ
影响因子：
4.4
作者：
Kato, Yasuhiko;Kobayashi, Kaoru;Iguchi, Taisen
通讯作者：
Iguchi, Taisen