Retrospective Analysis of Serotype Switching of Vibrio cholerae O1 in a Cholera Endemic Region Shows It Is a Non-random Process.
Retrospective Analysis of Serotype Switching of Vibrio cholerae O1 in a Cholera Endemic Region Shows It Is a Non-random Process.
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DOI：
10.1371/journal.pntd.0005044
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发表时间：
2016-10
影响因子：
3.8
通讯作者：
Lebens M
中科院分区：
文献类型：
--
作者：
Karlsson SL;Thomson N;Mutreja A;Connor T;Sur D;Ali M;Clemens J;Dougan G;Holmgren J;Lebens M
基因组数据产生的临床霍乱弧菌O1分离株收集了超过5年的时间在加尔各答，印度的季节性霍乱爆发的地区允许发生从小川到稻叶和小川的血清型转换的详细遗传分析。从小川到稻叶的变化是由wbeT基因编码的甲基转移酶突变破坏引起的。小川血清型的重新出现被认为是由于已经存在的小川进化枝的扩张或稻叶进化枝中突变的逆转。我们的数据表明，这种转变不是随机事件，而是由尚未确定的选择机制，基于O1抗原的结构差异或决定WBET基因的突变。霍乱是世界许多地区的一个主要健康问题，在流行地区造成季节性爆发。基本上只有O1血清群的霍乱弧菌引起流行性霍乱。该血清群有两种免疫学上可区分的血清型变体，称为Ogawa和Inaba。稻叶血清型是单一基因wbeT突变的结果，该基因以其完整形式编码使脂多糖侧链的末端perosamine糖甲基化的酶，从而导致Ogawa血清型。通过对流行区引起霍乱的细菌的遗传谱系进行为期五年的仔细检查，我们显示的数据表明血清型转换不是一个随机过程，而是由尚未确定的选择压力驱动的。
Genomic data generated from clinical Vibrio cholerae O1 isolates collected over a five year period in an area of Kolkata, India with seasonal cholera outbreaks allowed a detailed genetic analysis of serotype switching that occurred from Ogawa to Inaba and back to Ogawa. The change from Ogawa to Inaba resulted from mutational disruption of the methyltransferase encoded by the wbeT gene. Re-emergence of the Ogawa serotype was found to result either from expansion of an already existing Ogawa clade or reversion of the mutation in an Inaba clade. Our data suggests that such transitions are not random events but rather driven by as yet unidentified selection mechanisms based on differences in the structure of the O1 antigen or in the serotype-determining wbeT gene. Cholera is a major health problem in many parts of the world causing seasonal outbreaks in endemic areas. Essentially only the O1 serogroup of Vibrio cholerae causes epidemic cholera. This serogroup has two immunologically distinguishable serotype variants called Ogawa and Inaba. The Inaba serotype is a consequence of a mutation in a single gene, wbeT, that in its intact form encodes for an enzyme that methylates the terminal perosamine sugar of the lipopolysaccharide side chain thus resulting in the Ogawa serotype. By careful examination over a five-year period of the genetic lineages of bacteria causing cholera in an endemic area we show data indicating that serotype switching is not a random process but is driven by selection pressures that have yet to be identified.
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感染了Inaba或Ogawa血清型的脱水腹泻患者的临床特征和免疫学参数的比较。
影响因子：
--
作者：
Khan AI;Chowdhury F;Harris JB;Larocque RC;Faruque AS;Ryan ET;Calderwood SB;Qadri F
通讯作者：
Qadri F
影响因子：
11.8
作者：
Reimer AR;Van Domselaar G;Stroika S;Walker M;Kent H;Tarr C;Talkington D;Rowe L;Olsen-Rasmussen M;Frace M;Sammons S;Dahourou GA;Boncy J;Smith AM;Mabon P;Petkau A;Graham M;Gilmour MW;Gerner-Smidt P;V. cholerae Outbreak Genomics Task Force
通讯作者：
V. cholerae Outbreak Genomics Task Force
影响因子：
2.4
作者：
Sharifnia, A.;Bakhshi, B.;Pourshafie, M. R.
通讯作者：
Pourshafie, M. R.
影响因子：
--
作者：
Stine, O. Colin;Morris, J. Glenn, Jr.
通讯作者：
Morris, J. Glenn, Jr.
影响因子：
15.9
作者：
Reeves, PR;Hobbs, M;Rick, PD
通讯作者：
Rick, PD