Constitutive Expression of a Tomato Small Heat Shock Protein Gene LeHSP21 Improves Tolerance to High-Temperature Stress by Enhancing Antioxidation Capacity in Tobacco
Constitutive Expression of a Tomato Small Heat Shock Protein Gene LeHSP21 Improves Tolerance to High-Temperature Stress by Enhancing Antioxidation Capacity in Tobacco
复制标题
番茄小热激蛋白基因LeHSP21的组成型表达通过增强烟草的抗氧化能力来提高对高温胁迫的耐受性
DOI：
10.1007/s11105-015-0925-3
复制
发表时间：
2016-04
影响因子：
2.1
通讯作者：
Zhang Hongxia
中科院分区：
文献类型：
--
作者：
Zhang Jiang;Chen Haiyan;Wang Haihai;Li Bei;Yi Yanjun;Kong Fanjing;Liu Jiayao;Zhang Hongxia
小分子热休克蛋白（small heat shock proteins，sHSPs）是一类重要的热耐受蛋白，具有丰富的多样性。本研究以番茄（Lycopersicon esculentum cv PKM-1）叶绿体小分子热激蛋白基因（LeHSP 21）为材料，在烟草（Nicotiana tabacum L.）cv威斯康星州38）植株。当与野生型对照植物相比时，由花椰菜花叶病毒35 S启动子驱动的组成型表达LeHSP 21的转基因烟草植物表现出对高温和氧化胁迫的耐受性提高。高温处理后，转基因植株体内的抗氧化酶活性和脯氨酸含量显著高于野生型植株。我们的研究结果表明，通过表达LeHSP 21基因来提高植物的高温和氧化胁迫耐受性是可行的。
It is well established that small heat shock proteins (sHSPs) play an important role in thermotolerance in various organisms due to their abundance and diversity. In the present study, a chloroplast small heat shock protein gene (LeHSP21) from tomato (Lycopersicon esculentum cv PKM-1) was constitutively expressed in tobacco (Nicotiana tabacum L. cv Wisconsin 38) plants via Agrobacterium-mediated transformation. When compared to wild-type control plants, transgenic tobacco plants constitutively expressing LeHSP21, driven by the cauliflower mosaic virus 35S promoter, exhibited improved tolerance to both high temperature and oxidative stress. Furthermore, when the seedlings were subjected to high temperature treatment, the activities of anti-oxidative enzymes and the content of proline were significantly higher in transgenic plants than those in the wild-type plants. Our results presented here demonstrate the feasibility of improving high temperature and oxidative stress tolerance in plants through the expression of LeHSP21 gene.
登录
查看更多内容
杨属中成束蛋白样阿拉伯半乳聚糖蛋白FLA6基因的反义表达抑制其同源基因的表达并改变转基因树中的茎生物力学和细胞壁组成
影响因子：
6.9
作者：
Wang H;Jiang C;Wang C;Yang Y;Yang L;Gao X;Zhang H
通讯作者：
Zhang H
影响因子：
4.3
作者：
O. Lubaretz;U. zur Nieden
通讯作者：
O. Lubaretz;U. zur Nieden
影响因子：
1
作者：
A. Grover;P. Aggarwal;A. Kapoor;Surekha Katiyar-Agarwal;Manu Agarwal;Anupama Chandramouli
通讯作者：
A. Grover;P. Aggarwal;A. Kapoor;Surekha Katiyar-Agarwal;Manu Agarwal;Anupama Chandramouli
影响因子：
2.7
作者：
J. Šantrůček
通讯作者：
J. Šantrůček
影响因子：
3.5
作者：
Lee, BH;Won, SH;Jo, J
通讯作者：
Jo, J