Plant Rhizosphere Selection of Plasmodiophorid Lineages from Bulk Soil: The Importance of "Hidden" Diversity.
Plant Rhizosphere Selection of Plasmodiophorid Lineages from Bulk Soil: The Importance of "Hidden" Diversity.
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DOI：
10.3389/fmicb.2018.00168
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发表时间：
2018
影响因子：
5.2
通讯作者：
Bending GD
中科院分区：
文献类型：
--
作者：
Bass D;van der Gast C;Thomson S;Neuhauser S;Hilton S;Bending GD
与根际密切相关的微生物群落可以对植物的健康和生长产生强烈的积极和消极影响。采用群体特异性扩增子方法研究了油菜和小麦农业系统根际/根系和土壤样品中疟原虫多样性和分布的局部尺度驱动因素。疟原虫是植物和层桩相关的原生生物，包括众所周知的植物病原体和无症状的内源性物种。结果表明，小麦根际与OSR根际以及根际与土壤样品间的疟原虫群落差异很大，且存在显著差异。土壤体群落在盐砂还田和小麦还田系统间无显著差异。小麦和OSR根际选择不同的疟原虫系系。在OSR根际中，一个对应于nasturtii Spongospora的OTU被正选择，两个遗传上不同的OTU也被正选择。小麦根际样品中发现了两个与veronicae相关的新谱系，这表明植物与原生生物之间的关系尚不清楚。我们表明，微生物共生-宿主生态学的群体靶向eDNA方法揭示了显著的新多样性，并能够推断序列类型之间的差异活性和潜在相互作用，以及它们的存在。
Microbial communities closely associated with the rhizosphere can have strong positive and negative impacts on plant health and growth. We used a group-specific amplicon approach to investigate local scale drivers in the diversity and distribution of plasmodiophorids in rhizosphere/root and bulk soil samples from oilseed rape (OSR) and wheat agri-systems. Plasmodiophorids are plant- and stramenopile-associated protists including well known plant pathogens as well as symptomless endobiotic species. We detected 28 plasmodiophorid lineages (OTUs), many of them novel, and showed that plasmodiophorid communities were highly dissimilar and significantly divergent between wheat and OSR rhizospheres and between rhizosphere and bulk soil samples. Bulk soil communities were not significantly different between OSR and wheat systems. Wheat and OSR rhizospheres selected for different plasmodiophorid lineages. An OTU corresponding to Spongospora nasturtii was positively selected in the OSR rhizosphere, as were two genetically distinct OTUs. Two novel lineages related to Sorosphaerula veronicae were significantly associated with wheat rhizosphere samples, indicating unknown plant-protist relationships. We show that group-targeted eDNA approaches to microbial symbiont-host ecology reveal significant novel diversity and enable inference of differential activity and potential interactions between sequence types, as well as their presence.
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影响因子：
3.7
作者：
Hilton S;Bennett AJ;Keane G;Bending GD;Chandler D;Stobart R;Mills P
通讯作者：
Mills P
影响因子：
4.2
作者：
Cook, WRI;Schwartz, EJ
通讯作者：
Schwartz, EJ
影响因子：
3
作者：
Claxton, JR;Arnold, DL;Blakesley, D
通讯作者：
Blakesley, D
影响因子：
11
作者：
Chaparro, Jacqueline M.;Badri, Dayakar V.;Vivanco, Jorge M.
通讯作者：
Vivanco, Jorge M.
影响因子：
48
作者：
Edgar, Robert C.
通讯作者：
Edgar, Robert C.