Muscle-Enriched MicroRNAs Isolated from Whole Blood Are Regulated by Exercise and Are Potential Biomarkers of Cardiorespiratory Fitness.
Muscle-Enriched MicroRNAs Isolated from Whole Blood Are Regulated by Exercise and Are Potential Biomarkers of Cardiorespiratory Fitness.
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从全血中分离的富含肌肉的microRNA受运动调节，是心肺适应性的潜在生物标志物。
DOI：
10.3389/fgene.2016.00196
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发表时间：
2016
影响因子：
3.7
通讯作者：
Prestes PR
中科院分区：
文献类型：
--
作者：
Denham J;Prestes PR
MicroRNAs(MiRNAs)是一种小的非编码RNA分子，在转录后调节基因的表达。越来越多的证据表明，miRNAs影响运动诱导的健康和表现适应。循环中的miRNAs负责细胞间的通讯，可以作为疾病和运动相关特征的生物标志物。这些生物标志物将有助于运动筛选、监测和个性化运动处方的开发。因此，我们研究了长期剧烈有氧运动训练和单次最大有氧运动对五个与运动适应有关的肌肉丰富的miRNA(miR-1、miR-133a、miR-181a、miR-486和miR-494)的影响。我们还确定了miRNAs、静息心率和最大摄氧量(O2 Max)之间的线性相关性。采用TaqMan定量聚合酶链式反应检测67例耐力运动员和61例健康体检者静息血中miR-1、miR-133a、miR-181a、miR-486和miR-494的丰度。与对照组相比，耐力运动员表现出更高的miR-1、miR-486和miR-494含量(变化1.26到1.58倍，均P<；0.05)。在19名健康年轻男性(20.7±2.4岁)进行最大有氧运动后，miR-1、miR-133a和miR-486立即降低(0.64~0.76倍，均P<0.01)。最后，我们观察到miRNA丰度与O2 max(miR-1和miR-486)呈正相关，而miR-486与静息心率呈负相关。因此，从全血中分离出的富含肌肉的miRNAs受到急性和长期有氧运动训练的调节，可以作为心肺健康的生物标志物。
MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression post-transcriptionally. Evidence indicating miRNAs influence exercise-induced health and performance adaptations is mounting. Circulating miRNAs are responsible for intercellular communication and could serve as biomarkers for disease and exercise-related traits. Such biomarkers would contribute to exercise screening, monitoring, and the development of personalized exercise prescription. Accordingly, we investigated the impact of long-term strenuous aerobic exercise training and a single bout of maximal aerobic exercise on five muscle-enriched miRNAs implicated in exercise adaptations (miR-1, miR-133a, miR-181a, miR-486, and miR-494). We also determined linear correlations between miRNAs, resting heart rate, and maximum oxygen uptake (O2 max). We used TaqMan assay quantitative polymerase chain reaction to analyze the abundance of miR-1, miR-133a, miR-181a, miR-486, and miR-494 in resting whole blood of 67 endurance athletes and 61 healthy controls. Relative to controls, endurance athletes exhibited increased miR-1, miR-486, and miR-494 content (1.26- to 1.58-fold change, all p < 0.05). miR-1, miR-133a, and miR-486 were decreased immediately after maximal aerobic exercise (0.64- to 0.76-fold change, all p < 0.01) performed by 19 healthy, young men (20.7 ± 2.4 years). Finally, we observed positive correlations between miRNA abundance and O2 max (miR-1 and miR-486) and an inverse correlation between miR-486 and resting heart rate. Therefore, muscle-enriched miRNAs isolated from whole blood are regulated by acute and long-term aerobic exercise training and could serve as biomarkers of cardiorespiratory fitness.
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