Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
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SARS-CoV-2在一组实验室细胞系中的培养，以及生长特征的差异。
DOI：
10.1007/s10096-020-04106-0
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
La Scola B
中科院分区：
文献类型：
--
作者：
Wurtz N;Penant G;Jardot P;Duclos N;La Scola B
2019年底因SARS-CoV-2导致的COVID-19疾病的出现与VERO细胞共培养物中分离出的菌株迅速相关。这些分离很快使得进行抗病毒药物敏感性和药物再利用的第一次测试成为可能。然而，清点所有可以支持该病毒生长的细胞并评估分离株之间可能的差异似乎很重要。在目前的工作中，我们测试了4株SARS-CoV-2本地分离的34个细胞系在我们的实验室，通常用于人类病原微生物的分离。接种后，观察细胞的细胞病变效应，并使用定量实时聚合酶反应来测量细胞上的病毒复制。我们能够在7个细胞系、6个猿和人Caco-2上获得生长。致细胞病变效应是可变的，范围从48-72小时内的细胞单层溶解到无致细胞病变效应，尽管在Caco-2细胞中有强烈的增殖。有趣的是，根据测试的菌株，效果和增殖变化很大。在本文中，我们探讨了物种特异性和组织嗜性的SARS-CoV-2在体外的一组细胞在我们的实验室，并确定了人类和动物的细胞系容易支持SARS-CoV-2复制。我们的工作强调了在测试抗病毒分子和进行病理生理分析时测试多种菌株的重要性。
The emergence of COVID-19 disease due to SARS-CoV-2 at the end of 2019 was rapidly associated with the isolation of the strain from co-culture onto VERO cells. These isolations quickly made it possible to carry out the first tests for antiviral agents’ susceptibility and drug repurposing. However, it seems important to make an inventory of all the cells that can support the growth of this virus and evaluate possible differences between isolates. In the present work, we tested 4 strains of SARS-CoV-2 locally isolated on a panel of 34 cell lines present in our laboratory and commonly used for the isolation of human pathogenic microorganism. After inoculation, cells were observed for cytopathic effects and quantitative real-time polymerase reaction was used to measure the virus replication on the cells. We were able to obtain growth on 7 cell lines, 6 simian, and the human Caco-2. The cytopathogenic effects are variable, ranging from lysis of the cell monolayer in 48–72 h to no cytopathic effect in spite of intense multiplication, as in Caco-2 cells. Interestingly, effect and multiplication varied widely according to the strain tested. In this paper, we explored the species specificity and tissue tropism of SARS-CoV-2 in vitro on a panel of cells available in our laboratory and identified human and animal cell lines susceptible to support SARS-CoV-2 replication. Our work highlights the importance of testing multiple strains when testing antiviral molecules and performing patho-physiological analyzes.
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DOI：
10.1007/s10096-020-03913-9
发表时间：
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影响因子：
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作者：
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通讯作者：
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