Axonal connections between S1 barrel, M1, and S2 cortex in the newborn mouse.
Axonal connections between S1 barrel, M1, and S2 cortex in the newborn mouse.
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DOI：
10.3389/fnana.2023.1105998
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发表时间：
2023
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
初级（S1）、次级体感（S2）和运动（M1）皮质区域之间的功能互联网络的发展需要通过皮质皮质投射进行连贯的神经元活动。然而，在发育早期，S1和M1或S2之间的功能连接的解剖学基础仍然难以捉摸。在本研究中，我们在多聚甲醛固定的新生小鼠大脑中使用离体碳青碱染料（DiI）示踪，研究了S1桶区（S1Bf）、M1和S2不同层的神经元向亚板（SP）的轴突投射，亚板是未成熟皮层中感觉信息传递的中枢层。此外，我们在新皮层切片中进行了细胞外记录，以揭示这些区域之间的功能连接。我们的实验表明，来自皮质板（CP）、5/6层（L5/6）和M1和S2的SP的P0神经元已经通过S1Bf的SP发送投射。反过来，从CP到S1Bf的SP的神经元通过M1和S2的SP发送投射。皮质切片多电极阵列电生理记录显示，S1和M1内部和之间SP和L5/6之间的突触连接微弱，但功能正常。S1和S2之间的功能连通性甚至更低。总之，我们的研究结果表明，SP和上层皮质层之间的功能连接并不局限于同一皮质区域，但相邻皮质区域之间的皮质连接在出生之日就已经存在。因此，SP可以整合M1、S1和S2的早期皮质活动，并在早期塑造感觉运动整合的发展。
The development of functionally interconnected networks between primary (S1), secondary somatosensory (S2), and motor (M1) cortical areas requires coherent neuronal activity via corticocortical projections. However, the anatomical substrate of functional connections between S1 and M1 or S2 during early development remains elusive. In the present study, we used ex vivo carbocyanine dye (DiI) tracing in paraformaldehyde-fixed newborn mouse brain to investigate axonal projections of neurons in different layers of S1 barrel field (S1Bf), M1, and S2 toward the subplate (SP), a hub layer for sensory information transfer in the immature cortex. In addition, we performed extracellular recordings in neocortical slices to unravel the functional connectivity between these areas. Our experiments demonstrate that already at P0 neurons from the cortical plate (CP), layer 5/6 (L5/6), and the SP of both M1 and S2 send projections through the SP of S1Bf. Reciprocally, neurons from CP to SP of S1Bf send projections through the SP of M1 and S2. Electrophysiological recordings with multi-electrode arrays in cortical slices revealed weak, but functional synaptic connections between SP and L5/6 within and between S1 and M1. An even lower functional connectivity was observed between S1 and S2. In summary, our findings demonstrate that functional connections between SP and upper cortical layers are not confined to the same cortical area, but corticocortical connection between adjacent cortical areas exist already at the day of birth. Hereby, SP can integrate early cortical activity of M1, S1, and S2 and shape the development of sensorimotor integration at an early stage.
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DOI：
10.1016/s0165-3806(01)00130-4
发表时间：
2001-04-30
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Gaïarsa, JL;Khalilov, I;Ben-Ari, Y
通讯作者：
Ben-Ari, Y
影响因子：
5.8
作者：
Arganda-Carreras, Ignacio;Kaynig, Verena;Seung, H. Sebastian
通讯作者：
Seung, H. Sebastian
影响因子：
7.7
作者：
Dooley, James C.;Blumberg, Mark S.
通讯作者：
Blumberg, Mark S.
影响因子：
2.9
作者：
Luhmann HJ;Kilb W;Hanganu-Opatz IL
通讯作者：
Hanganu-Opatz IL
影响因子：
2.5
作者：
Choi, Jee Hyun;Koch, Klaus Peter;Shin, Hee-Sup
通讯作者：
Shin, Hee-Sup