Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes.
Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes.
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茶树角质层蒸腾屏障的可塑性对缺水和补给的响应：角质层蜡的作用
DOI：
10.3389/fpls.2020.600069
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发表时间：
2020
影响因子：
5.6
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Zhang Y;Du Z;Han Y;Chen X;Kong X;Sun W;Chen C;Chen M
角质层被视为非生命组织；目前尚不清楚角质层是否可以被可逆地改变以及潜在的机制是什么。在本研究中，对三种茶种质（乌牛枣、0202-10 和 0306A）进行缺水，然后再水化。从成熟的第 5 叶开始对两个叶表面的角质层外蜡和角质层内蜡进行定量。然后根据叶片干燥曲线测量角质层蒸腾速率，并分析角质层蒸腾速率与角质层蜡质覆盖率之间的相关性。我们发现，干旱会强化角质层蒸腾屏障，而补水处理则会逆转角质层蒸腾屏障。最初较弱的角质层蒸腾屏障优先因干旱胁迫而得到加强，而最初的主要角质层蒸腾屏障要么得到加强，要么没有改变。相关分析表明，角质层修饰可以通过多种机制将特定的蜡化合物选择性沉积到各个角质层隔室中来实现，包括体内蜡合成或运输、角质层外蜡和角质层内蜡之间的动态相分离、体外聚合以及逆向运输到表皮细胞壁或原生质体中以进一步转化。我们的数据表明，对来自各个角质层隔室的一组有限的特定蜡成分进行修改足以改变角质层的蒸腾屏障特性。
The cuticle is regarded as a non-living tissue; it remains unknown whether the cuticle could be reversibly modified and what are the potential mechanisms. In this study, three tea germplasms (Wuniuzao, 0202-10, and 0306A) were subjected to water deprivation followed by rehydration. The epicuticular waxes and intracuticular waxes from both leaf surfaces were quantified from the mature 5th leaf. Cuticular transpiration rates were then measured from leaf drying curves, and the correlations between cuticular transpiration rates and cuticular wax coverage were analyzed. We found that the cuticular transpiration barriers were reinforced by drought and reversed by rehydration treatment; the initial weak cuticular transpiration barriers were preferentially reinforced by drought stress, while the original major cuticular transpiration barriers were either strengthened or unaltered. Correlation analysis suggests that cuticle modifications could be realized by selective deposition of specific wax compounds into individual cuticular compartments through multiple mechanisms, including in vivo wax synthesis or transport, dynamic phase separation between epicuticular waxes and the intracuticular waxes, in vitro polymerization, and retro transportation into epidermal cell wall or protoplast for further transformation. Our data suggest that modifications of a limited set of specific wax components from individual cuticular compartments are sufficient to alter cuticular transpiration barrier properties.
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影响因子：
5.3
作者：
Hasanuzzaman M;Davies NW;Shabala L;Zhou M;Brodribb TJ;Shabala S
通讯作者：
Shabala S
影响因子：
5.6
作者：
Guzmán P;Fernández V;Graça J;Cabral V;Kayali N;Khayet M;Gil L
通讯作者：
Gil L
影响因子：
2.6
作者：
Hussain, Sajjad;Khalid, Muhammad Fasih;Anjum, Muhammad Akbar
通讯作者：
Anjum, Muhammad Akbar
影响因子：
7.3
作者：
Jetter, R;Schäffer, S;Riederer, M
通讯作者：
Riederer, M
影响因子：
6.5
作者：
Guzman, Paula;Fernandez, Victoria;Gil, Luis
通讯作者：
Gil, Luis