Airborne multidrug-resistant bacteria isolated from a concentrated swine feeding operation.
Airborne multidrug-resistant bacteria isolated from a concentrated swine feeding operation.
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DOI：
10.1289/ehp.7473
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发表时间：
2005-02
影响因子：
10.4
通讯作者：
Schwab K
中科院分区：
文献类型：
--
作者：
Chapin A;Rule A;Gibson K;Buckley T;Schwab K
在猪生产中使用非治疗水平的抗生素可以选择猪的共生菌和致病菌的抗生素耐药性。因此，零售猪肉产品以及被猪粪污染的地表水和地下水已被证明是人类接触耐抗生素细菌的来源。然而，目前尚不清楚养猪场内的空气是否也是暴露于耐抗生素细菌病原体的来源。为了研究这一问题，我们使用全玻璃冲击器对集中养猪作业中的空气进行了采样。采用分离肠球菌的方法对样品进行分析。采用标准生化试验对137株推定分离的肠球菌进行了种级鉴定，并用琼脂稀释法分析了对红霉素、克林霉素、维吉尼霉素、四环素和万古霉素的耐药性。其中34%为肠球菌，32%为凝固酶阴性葡萄球菌，33%为翠绿菌群链球菌。无论细菌种类如何，98%的分离株对至少两种猪生产中常用的抗生素表现出高度耐药性。这些分离株都没有对万古霉素（vancomycin）产生耐药性，万古霉素是一种在美国从未被批准用于牲畜的抗生素。综上所述，某集中养猪场空气中检出高水平耐多药肠球菌、凝固酶阴性葡萄球菌和翠绿菌群链球菌。这些发现表明，从这些设施吸入的空气可能是多重耐药细菌病原体从猪向人类转移的暴露途径。
The use of nontherapeutic levels of antibiotics in swine production can select for antibiotic resistance in commensal and pathogenic bacteria in swine. As a result, retail pork products, as well as surface and groundwaters contaminated with swine waste, have been shown to be sources of human exposure to antibiotic-resistant bacteria. However, it is unclear whether the air within swine operations also serves as a source of exposure to antibiotic-resistant bacterial pathogens. To investigate this issue, we sampled the air within a concentrated swine feeding operation with an all-glass impinger. Samples were analyzed using a method for the isolation of Enterococcus. A total of 137 presumptive Enterococcus isolates were identified to species level using standard biochemical tests and analyzed for resistance to erythromycin, clindamycin, virginiamycin, tetracycline, and vancomycin using the agar dilution method. Thirty-four percent of the isolates were confirmed as Enterococcus, 32% were identified as coagulase-negative staphylococci, and 33% were identified as viridans group streptococci. Regardless of bacterial species, 98% of the isolates expressed high-level resistance to at least two antibiotics commonly used in swine production. None of the isolates were resistant to vancomycin, an antibiotic that has never been approved for use in livestock in the United States. In conclusion, high-level multidrug-resistant Enterococcus, coagulase-negative staphylococci, and viridans group streptococci were detected in the air of a concentrated swine feeding operation. These findings suggest that the inhalation of air from these facilities may serve as an exposure pathway for the transfer of multidrug-resistant bacterial pathogens from swine to humans.
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影响因子：
9.8
作者：
Campagnolo, ER;Johnson, KR;McGeehin, M
通讯作者：
McGeehin, M
影响因子：
9.4
作者：
Gambarotto, K;Ploy, MC;Denis, F
通讯作者：
Denis, F
影响因子：
9.4
作者：
Donabedian, SM;Thal, LA;Zervos, MJ
通讯作者：
Zervos, MJ
影响因子：
10.4
作者：
Hamscher G;Pawelzick HT;Sczesny S;Nau H;Hartung J
通讯作者：
Hartung J
DOI：
10.1080/15298669191364721
发表时间：
1991-07-01
期刊：
AMERICAN INDUSTRIAL HYGIENE ASSOCIATION JOURNAL
影响因子：
--
作者：
CROOK, B;ROBERTSON, JF;TOPPING, MD
通讯作者：
TOPPING, MD