Three-dimensional finite element modeling of pericellular matrix and cell mechanics in the nucleus pulposus of the intervertebral disk based on in situ morphology.
Three-dimensional finite element modeling of pericellular matrix and cell mechanics in the nucleus pulposus of the intervertebral disk based on in situ morphology.
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DOI：
10.1007/s10237-010-0214-x
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发表时间：
2011-02
影响因子：
3.5
通讯作者：
Setton, Lori A.
中科院分区：
文献类型：
--
作者：
Cao, Li;Guilak, Farshid;Setton, Lori A.
椎间盘（IVD）的髓核（NP）细胞具有独特的形态学特征和对机械刺激的生物学反应，可以调节IVD的维持和健康。NP细胞以单细胞、成对或多细胞的形式存在于连续的细胞周基质（PCM）中，其结构和性质可显著影响细胞和细胞外基质的力学。在这项研究中，开发了一个计算模型来预测的应力-应变，流体压力和流场的细胞及其周围的PCM在NP使用三维（3D）有限元模型的基础上原位形态的成熟大鼠NP的细胞PCM区域，使用共聚焦显微镜测量。细胞外基质和代表性细胞基质单位的三维几何形状被用来构建各向同性和双相材料的结构的三维有限元模型。响应于细胞外基质的压缩应变，预测NP细胞和PCM区域分别经历比细胞外基质大1.9-3.7倍和1.4-2.1倍的体积应变。体积和偏应变集中通常被发现在细胞/PCM接口，而冯米塞斯应力集中与PCM/细胞外基质接口。细胞基质单位含有更大的细胞数量与较高的峰值细胞株和较低的液体加压率后加载。这些研究为NP细胞的微观力学提供了新的模型预测，有助于理解IVD中的机械转导及其随衰老和退化的变化。
Nucleus pulposus (NP) cells of the intervertebral disk (IVD) have unique morphological characteristics and biologic responses to mechanical stimuli that may regulate maintenance and health of the IVD. NP cells reside as single cell, paired or multiple cells in a contiguous pericellular matrix (PCM), whose structure and properties may significantly influence cell and extracellular matrix mechanics. In this study, a computational model was developed to predict the stress–strain, fluid pressure and flow fields for cells and their surrounding PCM in the NP using three-dimensional (3D) finite element models based on the in situ morphology of cell–PCM regions of the mature rat NP, measured using confocal microscopy. Three-dimensional geometries of the extracellular matrix and representative cell–matrix units were used to construct 3D finite element models of the structures as isotropic and biphasic materials. In response to compressive strain of the extracellular matrix, NP cells and PCM regions were predicted to experience volumetric strains that were 1.9–3.7 and 1.4–2.1 times greater than the extracellular matrix, respectively. Volumetric and deviatoric strain concentrations were generally found at the cell/PCM interface, while von Mises stress concentrations were associated with the PCM/extracellular matrix interface. Cell–matrix units containing greater cell numbers were associated with higher peak cell strains and lower rates of fluid pressurization upon loading. These studies provide new model predictions for micromechanics of NP cells that can contribute to an understanding of mechanotransduction in the IVD and its changes with aging and degeneration.
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影响因子：
2.8
作者：
ISHII, T;TSUJI, H;TERAHATA, N
通讯作者：
TERAHATA, N
影响因子：
2.4
作者：
Alexopoulos, LG;Williams, GM;Guilak, F
通讯作者：
Guilak, F
影响因子：
3
作者：
Johannessen, W;Elliott, DM
通讯作者：
Elliott, DM
影响因子：
3.5
作者：
Haider, MA;Schugart, RC;Guilak, F
通讯作者：
Guilak, F
DOI：
10.1115/1.429655
发表时间：
2000-06-01
影响因子：
1.7
作者：
Baer, AE;Setton, LA
通讯作者：
Setton, LA