Increasing mechanical strength of gelatin hydrogels by divalent metal ion removal.
Increasing mechanical strength of gelatin hydrogels by divalent metal ion removal.
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DOI：
10.1038/srep04706
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发表时间：
2014-04-16
影响因子：
4.6
通讯作者：
Zhao F
中科院分区：
文献类型：
--
作者：
Xing Q;Yates K;Vogt C;Qian Z;Frost MC;Zhao F
由于明胶水凝胶的不稳定性和力学性能差，特别是在生理条件下，明胶水凝胶的应用受到限制。明胶中存在的二价金属离子如钙、铁在明胶分子间的相互作用中起着重要作用。本研究的目的是确定去除二价离子对明胶凝胶稳定性和机械性能的影响。明胶溶液经CHILEX树脂纯化后，用钠离子置换二价金属离子。然后用1-乙基-3-(3-二甲氨基丙基)碳二亚胺(EDC)对凝胶进行化学交联。结果表明，二价金属离子的去除对明胶网络的形成有显著影响。纯化后的明胶水凝胶与明胶分子间的相互作用较小，形成了较大的孔道网络，使EDC能够更有效地渗透和交联明胶。经交联的纯化凝胶具有较小的溶胀度、较高的交联度和较大的储损模数。二价离子的去除是一种简单而有效的方法，可以显著提高明胶水凝胶的稳定性和强度。体外细胞培养表明，纯化的明胶保持了支持细胞贴壁和铺展的能力。
The usage of gelatin hydrogel is limited due to its instability and poor mechanical properties, especially under physiological conditions. Divalent metal ions present in gelatin such as Ca2+ and Fe2+ play important roles in the gelatin molecule interactions. The objective of this study was to determine the impact of divalent ion removal on the stability and mechanical properties of gelatin gels with and without chemical crosslinking. The gelatin solution was purified by Chelex resin to replace divalent metal ions with sodium ions. The gel was then chemically crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). Results showed that the removal of divalent metal ions significantly impacted the formation of the gelatin network. The purified gelatin hydrogels had less interactions between gelatin molecules and form larger-pore network which enabled EDC to penetrate and crosslink the gel more efficiently. The crosslinked purified gels showed small swelling ratio, higher crosslinking density and dramatically increased storage and loss moduli. The removal of divalent ions is a simple yet effective method that can significantly improve the stability and strength of gelatin hydrogels. The in vitro cell culture demonstrated that the purified gelatin maintained its ability to support cell attachment and spreading.
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影响因子：
3
作者：
Saarai, Amarjargal;Sedlacek, Tomas;Saha, Petr
通讯作者：
Saha, Petr
DOI：
10.1111/j.1749-6632.2002.tb03041.x
发表时间：
2002-01-01
期刊：
REPARATIVE MEDICINE: GROWING TISSUES AND ORGANS
影响因子：
--
作者：
Godbey, WT;Atala, A
通讯作者：
Atala, A
影响因子：
3.4
作者：
Dragusin, Diana-Maria;Van Vlierberghe, Sandra;Stancu, Izabela-Cristina
通讯作者：
Stancu, Izabela-Cristina
DOI：
10.1016/j.ijbiomac.2005.03.012
发表时间：
2005-07-01
影响因子：
8.2
作者：
Mohanty, B;Bohidar, HB
通讯作者：
Bohidar, HB
影响因子：
2.8
作者：
Hong, Soyoung;Song, Seung-Joon;Park, Yongdoo
通讯作者：
Park, Yongdoo