Abnormal expression of SLIT3 induces intravillous vascularization dysplasia in ectopic pregnancy.
Abnormal expression of SLIT3 induces intravillous vascularization dysplasia in ectopic pregnancy.
复制标题
SLIT3异常表达导致宫外孕绒毛内血管发育不良
作者：
Zhu, Qian;Zhao, Xiaoya;Zhang, Duo;Xia, Wei;Zhang, Jian
研究异位妊娠（EP）绒毛的形态、毛细血管数量和转录组表达谱是否与正常妊娠（NP）绒毛不同。对 CD31 进行苏木精-伊红 (HE) 和免疫组织化学 (IHC) 染色，以比较 EP 和 NP 绒毛之间形态和毛细血管数量的差异。通过两种类型绒毛的转录组测序确定差异表达 (DE) miRNA 和 mRNA，并用于构建 miRNA-mRNA 网络，从中鉴定出枢纽基因。通过定量逆转录 (qRT)-PCR 验证了候选 DE-miRNA 和 DE-mRNA。确定了毛细血管数量和血清 β 人绒毛膜促性腺激素 (β-HCG) 水平之间以及与血管生成相关的枢纽基因的表达水平和 β-HCG 水平之间的相关性。与 NP 绒毛相比，EP 胎盘绒毛的平均横截面积和总横截面积显着增加。 EP绒毛中毛细血管密度大大降低，且与β-HCG水平呈正相关。从测序数据中总共鉴定出 49 个 DE-miRNA 和 625 个 DE-mRNA。综合分析建立了包含 32 个 DE-miRNA 和 103 个 DE-mRNA 的 miRNA-mRNA 网络。基于网络中hub mRNA和miRNA的验证，发现了一条涉及miR-491-5p–SLIT3的调控通路，该通路可能在绒毛毛细血管的发育中发挥作用。 EP 胎盘中绒毛形态、毛细血管数量和绒毛组织中 miRNA/mRNA 表达谱存在异常。具体而言，受miR-491-5p调节的SLIT3可能有助于绒毛血管生成的调节，并被确立为绒毛膜绒毛发育的推定预测因子，为未来的研究奠定了基础。
To investigate whether the morphology, capillary number, and transcriptome expression profiles of ectopic pregnancy (EP) villi differ from those of normal pregnancy (NP) villi. Hematoxylin-eosin (HE) and immunohistochemistry (IHC) staining for CD31 were conducted to compare differences in morphology and capillary number between EP and NP villi. Differentially expressed (DE) miRNAs and mRNAs were determined from transcriptome sequencing of both types of villi and used to construct a miRNA–mRNA network, from which hub genes were identified. Candidate DE-miRNAs and DE-mRNAs were validated by quantitative reverse transcription (qRT)-PCR. Correlations were identified between the number of capillaries and serum beta human chorionic gonadotropin (β-HCG) levels and between the expression levels of hub genes associated with angiogenesis and β-HCG levels. The mean and total cross-sectional areas of placental villi were significantly increased in EP compared with NP villi. Capillary density was greatly reduced in EP villi and was positively correlated with β-HCG levels. A total of 49 DE-miRNAs and 625 DE-mRNAs were identified from the sequencing data. An integrated analysis established a miRNA–mRNA network containing 32 DE-miRNAs and 103 DE-mRNAs. Based on the validation of hub mRNAs and miRNAs in the network, a regulatory pathway involving miR-491-5p–SLIT3 was discovered, which may have a role in the development of villous capillaries. Villus morphology, capillary number, and miRNA/mRNA expression profiles in villous tissues were aberrant in EP placentas. Specifically, SLIT3, which is regulated by miR-491-5p, may contribute to the regulation of villous angiogenesis and was established as a putative predictor of chorionic villus development, providing a basis for future research.
登录
查看更多内容
HE 和免疫组织化学染色评价大鼠股骨脱钙技术
影响因子：
--
作者：
Liu H;Zhu R;Liu C;Ma R;Wang L;Chen B;Li L;Niu J;Zhao D;Mo F;Fu M;Brömme D;Zhang D;Gao S
通讯作者：
Gao S
胎盘血管生成中缝隙/机器人信号传导的观点。
影响因子：
2
作者：
Liao WX;Wing DA;Geng JG;Chen DB
通讯作者：
Chen DB
影响因子：
4
作者：
Kokawa, K;Shikone, T;Nakano, R
通讯作者：
Nakano, R
DOI：
10.1111/j.1471-0528.2006.00882.x
发表时间：
2006-05-01
影响因子：
5.8
作者：
Egbor, M;Ansari, T;Sibbons, PD
通讯作者：
Sibbons, PD
影响因子：
1.2
作者：
Anselmo, MA;Dalvin, S;Kinane, TB
通讯作者：
Kinane, TB