Magnetic Resonance Imaging of Pathogenic Protozoan Parasite Entamoeba histolytica Labeled With Superparamagnetic Iron Oxide Nanoparticles
Magnetic Resonance Imaging of Pathogenic Protozoan Parasite Entamoeba histolytica Labeled With Superparamagnetic Iron Oxide Nanoparticles
复制标题
超顺磁性氧化铁纳米颗粒标记的致病性原生动物寄生虫溶组织内阿米巴的磁共振成像
DOI：
10.1097/rli.0000000000000175
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发表时间：
2015
影响因子：
6.7
通讯作者：
Lotter H.
中科院分区：
文献类型：
--
作者：
Fehling;Bernin;Zaruba;Bruchhaus;Ittrich;Lotter H.
ObjectivesThe的目的是建立一个非侵入性的跟踪病原性寄生虫溶组织内阿米巴（Eh）后，超顺磁性氧化铁（SPIO）标记的磁共振成像（MRI）在体外和体内的单细胞水平在小鼠模型阿米巴肝脓肿（ALA）。材料和方法当地机构审查委员会对动物护理批准的所有动物实验。用SPIO纳米颗粒（SPIO-Eh）标记溶组织内阿米巴滋养体。使用流式细胞术优化了Eh对SPIO的摄取，并通过明场、荧光和透射电子显微镜观察。通过测定红细胞的生长和摄取率来评估SPIO-Eh的体外活力。在临床前7 T MRI系统中使用连续重复的MRI扫描通过体外MRI证实SPIO-Eh的迁移。SPIO-Eh在小鼠肝脏内的体内分布进行了定性和定量评估，由连续呼吸触发的T2* 加权MRI，T2加权MRI，和R2* MR弛豫法注射后5天，并与免疫组织学的肝脏切片后removed.ResultsEntamoeba histolytica可以有效地标记SPIO寄生虫的生长速度或吞噬能力没有影响。体外动态MRI允许实时迁移监测和单个SPIO-Eh的速度测定。在体内，SPIO-Eh在ALA形成中显示T2* 加权信号降低和R2* 增加。SPIO-Eh的运动是必要的，以诱导ALA formations.ConclusionsThe本研究证明了一个有效的磁标记和非侵入性的体外和体内MR跟踪的致病性原生动物Eh在小鼠模型ALA的可行性，从而代表在未来的非侵入性成像工具来研究寄生虫，以及主机特定的病理机制。
ObjectivesThe aim of this study was to establish a noninvasive tracking of the pathogenic parasite Entamoeba histolytica (Eh) after superparamagnetic iron oxide (SPIO) labeling by magnetic resonance imaging (MRI) on a single-cell level in vitro and in vivo in a mouse model for amebic liver abscess (ALA).Materials and MethodsLocal institutional review committee on animal care approved all animal experiments. Entamoeba histolytica trophozoites were labeled with SPIO nanoparticles (SPIO-Eh). The uptake of SPIO by Eh was optimized using flow cytometry and visualized by bright field, fluorescence, and transmission electron microscopy. The viability of SPIO-Eh was assessed in vitro by determination of growth and ingestion rate of red blood cells. Migration of SPIO-Eh was proven by in vitro MRI in a preclinical 7 T MRI system using continually repeated MRI scans. In vivo distribution of SPIO-Eh within the mouse liver was assessed qualitatively and quantitatively by serial respiration-triggered T2*-weighted MRI, T2-weighted MRI, and R2* MR relaxometry up to 5 days after injection and correlated with immunohistology of the liver sections after removal.ResultsEntamoeba histolytica can be efficiently labeled with SPIO without influence on parasite growth rate or phagocytic capacity. In vitro dynamic MRI allowed real-time migration monitoring and determination of velocity of single SPIO-Eh. In vivo SPIO-Eh showed signal decrease in T2*-weighted and increase of R2* in ALA formations. Motility of SPIO-Eh was necessary to induce ALA formations.ConclusionsThe present study demonstrates the feasibility of an efficient magnetic labeling and a noninvasive in vitro and in vivo MR tracking of the pathogenic protozoan Eh in a mouse model for ALA, thus representing in future a noninvasive imaging tool to study parasite, as well as on host-specific pathomechanisms.
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超顺磁性氧化颗粒：Aktueller Stand und zukünftige Entwicklungen
DOI：
10.1055/s-2003-39935
发表时间：
2003
期刊：
RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der Bildgebenden Verfahren
影响因子：
--
作者：
M. Taupitz;S. Schmitz;B. Hamm
通讯作者：
B. Hamm
用氧化铁标记为大鼠模型中的粒细胞的软组织感染的磁共振成像。
影响因子：
3.7
作者：
Baraki H;Zinne N;Wedekind D;Meier M;Bleich A;Glage S;Hedrich HJ;Kutschka I;Haverich A
通讯作者：
Haverich A
影响因子：
4.4
作者：
Ittrich, Harald;Lange, Claudia;Nolte-Ernsting, Claus
通讯作者：
Nolte-Ernsting, Claus
影响因子：
6.2
作者：
Cahill, KS;Gaidosh, G;Walter, GA
通讯作者：
Walter, GA
影响因子：
19.7
作者：
Lutz, AM;Weishaupt, D;Kaim, AH
通讯作者：
Kaim, AH