Self-Collection Blood Test for PFASs: Comparing Volumetric Microsamplers with a Traditional Serum Approach.
Self-Collection Blood Test for PFASs: Comparing Volumetric Microsamplers with a Traditional Serum Approach.
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PFASs的自我采集血液检测：体积微采样与传统血清方法的比较。
DOI：
10.1021/acs.est.2c09852
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发表时间：
2023-05-30
影响因子：
11.4
通讯作者：
Higgins, Christopher P.
中科院分区：
文献类型：
--
作者：
Carignan, Courtney C.;Bauer, Rachel A.;Patterson, Andrew;Phomsopha, Thep;Redman, Eric;Stapleton, Heather M.;Higgins, Christopher P.
Eurofins开发了一种远程采样方法，用于定量使用体积吸收微量采样器（VAMS）采集的全血样本中的全氟烷基和多氟烷基物质（PFAS），该采样器允许使用手指穿刺自行采集血液。本研究比较了使用VAMSs自行采血测量的PFAS暴露量与标准静脉血清方法。从先前PFAS饮用水污染的社区中的参与者（n = 53）中使用静脉抽血以及使用VAMS的参与者自我收集血液样本。还将来自静脉管的全血加载到VAMS上，以比较毛细血管与静脉全血PFAS水平的差异。使用液相色谱串联质谱和在线固相萃取对样品进行PFASs定量。血清PFAS水平与毛细血管VAMS测定值高度相关（r ≥ 0.91和p < 0.05）。血清PFAS水平通常比全血高两倍，反映了其组成的预期差异。值得注意的是，FOSA在全血（静脉和毛细血管VAMS）中检出，但在血清中未检出。总体而言，这些研究结果表明，VAMS是评估人类暴露于PFAS升高的有用的自我收集工具。体积吸收式微量进样器利用毛细作用将血液抽入进样装置，是测量血液中PFAS升高的一种有用的自收集工具。
A remote sampling approach was developed at Eurofins for quantifying per- and polyfluoroalkyl substances (PFASs) in whole blood samples collected using volumetric absorptive microsamplers (VAMSs), which allow for self-collection of blood using a finger prick. This study compares PFAS exposure measured by self-collection of blood using VAMSs to the standard venous serum approach. Blood samples were collected from participants (n = 53) in a community with prior PFAS drinking water contamination using a venous blood draw as well as participant self-collection using VAMSs. Whole blood from the venous tubes was also loaded onto VAMSs to compare differences in capillary vs venous whole blood PFAS levels. Samples were quantified for PFASs using liquid chromatography tandem mass spectrometry and online solid-phase extraction. PFAS levels in serum were highly correlated with measurements in capillary VAMSs (r ≥ 0.91 and p < 0.05). Serum PFAS levels were generally twofold higher than whole blood, reflecting expected differences in their composition. Of interest, FOSA was detected in whole blood (both venous and capillary VAMSs) but not in serum. Overall, these findings indicate that VAMSs are useful self-collection tools for assessing elevated human exposure to PFASs. Volumetric absorptive microsamplers use capillary action to draw blood into the sampling device and can be a useful self-collection tool for measuring elevated PFAS in blood.
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影响因子：
7.9
作者：
Petrick LM;Arora M;Niedzwiecki MM
通讯作者：
Niedzwiecki MM
影响因子：
8.3
作者：
Barr DB;Kannan K;Cui Y;Merrill L;Petrick LM;Meeker JD;Fennell TR;Faustman EM
通讯作者：
Faustman EM
DOI：
10.1016/j.ijheh.2007.01.024
发表时间：
2007-05-01
影响因子：
6
作者：
Angerer, Juergen;Ewers, Ulrich;Wilhelm, Michael
通讯作者：
Wilhelm, Michael
影响因子：
6.2
作者：
Kato, Kayoko;Wanigatunga, Amal A.;Calafat, Antonia M.
通讯作者：
Calafat, Antonia M.
影响因子：
11.4
作者：
Poothong, Somrutai;Thomsen, Cathrine;Haug, Line Smastuen
通讯作者：
Haug, Line Smastuen