Phenotypes of Aging Postovulatory Oocytes After Somatic Cell Nuclear Transfer in Mice.
Phenotypes of Aging Postovulatory Oocytes After Somatic Cell Nuclear Transfer in Mice.
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小鼠体细胞核移植后排卵后卵母细胞老化的表型。
DOI：
10.1089/cell.2016.0014
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发表时间：
2016
期刊：
影响因子：
1.6
通讯作者：
Kishigami S.
中科院分区：
文献类型：
--
作者：
Lee AR;Shimoike T;Wakayama T;Kishigami S.
排卵后卵母细胞迅速失去发育潜力，称为排卵后卵母细胞老化，并且经常表现出特征表型，例如细胞碎片、纺锤体形状异常和染色体错位。在这里，我们利用体细胞核移植（SCNT）重建了小鼠卵母细胞，以揭示体细胞来源的细胞核对衰老过程中卵母细胞生理学的影响。正常卵母细胞在卵母细胞采集后24小时开始进行细胞破碎；然而，这种情况在 SCNT 卵母细胞中发生得更早，并且在 48 小时时更为严重，表明转移的体细胞核影响了卵母细胞的生理机能。我们发现正常卵母细胞和 SCNT 老化卵母细胞之间乙酰化 α-微管蛋白 (Ac-Tub) 和 α-微管蛋白 (Tub) 的状态没有差异，但与正常卵母细胞不同，老化 SCNT 卵母细胞没有星体微管。有趣的是，老化的 SCNT 卵母细胞表现出更严重的染色体分散或纺锤体形状不规则。对微丝的观察表明，在正常卵母细胞中，0小时时质膜下方有清晰的肌动蛋白环，纺锤体（肌动蛋白帽）周围有浓缩的微丝，1小时时肌动蛋白丝开始退化，24小时时完全破坏并分布到细胞质中。相比之下，在 SCNT 卵母细胞中，在 SCNT 后 1 小时内，移植细胞核周围形成肌动蛋白帽，并在 24 小时内仍然存在。因此，SCNT卵母细胞以相似但不同的方式老化，表明它们不仅含有表观遗传学异常的细胞核，而且在生理上也不同。
Oocytes rapidly lose their developmental potential after ovulation, termed postovulatory oocyte aging, and often exhibit characteristic phenotypes, such as cytofragmentation, abnormal spindle shapes, and chromosome misalignments. Here, we reconstructed mouse oocytes using somatic cell nuclear transfer (SCNT) to reveal the effect of somatic cell-derived nuclei on oocyte physiology during aging. Normal oocytes started undergoing cytofragmentation 24 hours after oocyte collection; however, this occurred earlier in SCNT oocytes and was more severe at 48 hours, suggesting that the transferred somatic cell nuclei affected oocyte physiology. We found no difference in the status of acetylated α-tubulin (Ac-Tub) and α-tubulin (Tub) between normal and SCNT aging oocytes, but unlike normal oocytes, aging SCNT oocytes did not have astral microtubules. Interestingly, aging SCNT oocytes displayed more severely scattered chromosomes or irregularly shaped spindles. Observations of the microfilaments showed that, in normal oocytes, there was a clear actin ring beneath the plasma membrane and condensed microfilaments around the spindle (the actin cap) at 0 hours, and the actin filaments started degenerating at 1 hour, becoming completely disrupted and distributed to the cytoplasm at 24 hours. By contrast, in SCNT oocytes, an actin cap formed around the transplanted nuclei within 1 hour of SCNT, which was still present at 24 hours. Thus, SCNT oocytes age in a similar but distinct way, suggesting that they not only contain nuclei with abnormal epigenetics but are also physiologically different.
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影响因子：
2.7
作者：
SCHATTEN, G;SIMERLY, C;SCHATTEN, H
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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DOI：
10.1002/mrd.1120190409
发表时间：
1988
期刊：
Gamete research
影响因子：
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作者：
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影响因子：
6.7
作者：
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