Spatially-interactive biomolecular networks organized by nucleic acid nanostructures.
Spatially-interactive biomolecular networks organized by nucleic acid nanostructures.
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DOI：
10.1021/ar200295q
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发表时间：
2012-08-21
影响因子：
18.3
通讯作者：
Yan, Hao
中科院分区：
文献类型：
--
作者：
Fu, Jinglin;Liu, Minghui;Liu, Yan;Yan, Hao
生命系统已经进化出多种纳米结构来控制介导许多功能的分子相互作用，包括受体对靶标的识别、酶与底物的结合以及酶活性的调节。在细胞外模仿这些结构需要对单个网络组件的组织进行纳米级控制的方法。DNA纳米技术的进步使得能够设计和制造复杂的一维、二维和三维（1D、2D和3D）纳米结构，其利用自发和序列特异性DNA杂交。与其他自组装生物聚合物相比，DNA纳米结构提供了可预测和可编程的相互作用，以及其他纳米颗粒和生物分子可以精确定位的表面特征。在构建高度组织的网络的同时控制组件的空间布置的能力将导致这些系统的各种应用。例如，具有分子探针的表面展示的DNA纳米阵列可以感测与DNA、RNA和蛋白质的非共价杂交相互作用以及共价化学反应。DNA纳米结构还可以将外部分子排列成定义明确的阵列，这可以提高许多结构确定方法的分辨率，例如X射线衍射，cryo-EM，NMR和超分辨率荧光。此外，通过将目标实体限制为特定构象，自组装DNA纳米结构可以作为分子标尺来评估构象依赖的活性。该帐户描述了DNA纳米结构定向组装生物分子网络的最新进展，并探讨了将该技术应用于其他研究领域的可能性。最近，一些报道已经证明了DNA纳米结构定向组装的空间相互作用的生物分子网络。例如，研究人员已经通过在体外使用DNA纳米支架或通过在体内利用RNA基质组织组分的位置来构建合成的多酶级联。与相应的非结构化酶混合物相比，这些结构显示出增强的效率。这种系统被设计成模拟细胞功能，其中酶之间的底物扩散被促进，并且反应以高效率和特异性被催化。此外，研究人员还使用DNA纳米支架将多个色素团组装成阵列，该支架优化了染料之间的相对距离及其空间组织。由此产生的人工光捕获系统表现出高效的级联能量传递。最后，DNA纳米结构已被用作组装模板，以构建执行合理设计的行为的纳米器件，包括货物装载，运输和路线控制。
Living systems have evolved a variety of nanostructures to control the molecular interactions that mediate many functions including the recognition of targets by receptors, the binding of enzymes to substrates, and the regulation of enzymatic activity. Mimicking these structures outside of the cell requires methods that offer nanoscale control over the organization of individual network components. Advances in DNA nanotechnology have enabled the design and fabrication of sophisticated one-, two- and three-dimensional (1D, 2D and 3D) nanostructures that utilize spontaneous and sequence specific DNA hybridization. Compared to other self-assembling biopolymers, DNA nanostructures offer predictable and programmable interactions, and surface features to which other nanoparticles and bio-molecules can be precisely positioned. The ability to control the spatial arrangement of the components while constructing highly-organized networks will lead to various applications of these systems. For example, DNA nanoarrays with surface displays of molecular probes can sense noncovalent hybridization interactions with DNA, RNA, and proteins and covalent chemical reactions. DNA nanostructures can also align external molecules into well-defined arrays, which may improve the resolution of many structural determination methods, such as X-ray diffraction, cryo-EM, NMR, and super-resolution fluorescence. Moreover, by constraining target entities to specific conformations, self-assembled DNA nanostructures can serve as molecular rulers to evaluate conformation-dependent activities. This Account describes the most recent advances in the DNA nanostructure directed assembly of biomolecular networks and explores the possibility of applying this technology to other fields of study. Recently, several reports have demonstrated the DNA nanostructure directed assembly of spatially-interactive biomolecular networks. For example, researchers have constructed synthetic multi-enzyme cascades by organizing the position of the components using DNA nanoscaffolds in vitro, or by utilizing RNA matrices in vivo. These structures display enhanced efficiency compared to the corresponding unstructured enzyme mixtures. Such systems are designed to mimic cellular function, where substrate diffusion between enzymes is facilitated and reactions are catalyzed with high efficiency and specificity. In addition, researchers have assembled multiple choromophores into arrays using a DNA nanoscaffold that optimizes the relative distance between the dyes and their spatial organization. The resulting artificial light harvesting system exhibits efficient cascading energy transfers. Finally, DNA nanostructures have been used as assembly templates to construct nanodevices that execute rationally-designed behaviors, including cargo loading, transportation and route control.
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影响因子：
14.9
作者：
Douglas SM;Marblestone AH;Teerapittayanon S;Vazquez A;Church GM;Shih WM
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