siRNA-mediated gene targeting in Aedes aegypti embryos reveals that frazzled regulates vector mosquito CNS development.
siRNA-mediated gene targeting in Aedes aegypti embryos reveals that frazzled regulates vector mosquito CNS development.
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DOI：
10.1371/journal.pone.0016730
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发表时间：
2011-01-31
期刊：
影响因子：
3.7
通讯作者：
Duman-Scheel M
中科院分区：
文献类型：
--
作者：
Clemons A;Haugen M;Le C;Mori A;Tomchaney M;Severson DW;Duman-Scheel M
尽管蚊子基因组计划发现了许多已知发育调控基因的直系同源物，但对媒介蚊子的发育知之甚少。在这里，我们调查的Netrin受体frazzled（FRA）胚胎神经索发育过程中的两个载体蚊子物种的作用。就在埃及伊蚊（登革热媒介）和冈比亚按蚊（疟疾媒介）的胚胎腹神经索的轴突发生之前和期间，在神经元中检测到FRA表达。通过SiRNA介导的Ae基因敲减研究了fra的功能分析。埃及胚胎在整个胚胎发生过程中维持的fra敲低的确认表明，显微注射siRNA是研究Ae中基因功能的有效方法。埃及胚胎在Ae期间的Fra损失。埃及人的发育导致连合轴突变薄和缺失。这些缺陷在性质上类似于在黑腹博士fra无效突变体中观察到的缺陷。然而，Aa。埃及伊蚊敲除表型更强，与果蝇无连合突变表型相似。这项调查的结果，第一次有针对性的基因敲除载体蚊子胚胎发育过程中，表明，虽然Fra发挥了关键作用，在发展的Ae。埃及腹神经索，调节胚胎连合轴突引导的机制已经在远亲昆虫中进化。
Although mosquito genome projects uncovered orthologues of many known developmental regulatory genes, extremely little is known about the development of vector mosquitoes. Here, we investigate the role of the Netrin receptor frazzled (fra) during embryonic nerve cord development of two vector mosquito species. Fra expression is detected in neurons just prior to and during axonogenesis in the embryonic ventral nerve cord of Aedes aegypti (dengue vector) and Anopheles gambiae (malaria vector). Analysis of fra function was investigated through siRNA-mediated knockdown in Ae. aegypti embryos. Confirmation of fra knockdown, which was maintained throughout embryogenesis, indicated that microinjection of siRNA is an effective method for studying gene function in Ae. aegypti embryos. Loss of fra during Ae. aegypti development results in thin and missing commissural axons. These defects are qualitatively similar to those observed in Dr. melanogaster fra null mutants. However, the Aa. aegypti knockdown phenotype is stronger and bears resemblance to the Drosophila commissureless mutant phenotype. The results of this investigation, the first targeted knockdown of a gene during vector mosquito embryogenesis, suggest that although Fra plays a critical role during development of the Ae. aegypti ventral nerve cord, mechanisms regulating embryonic commissural axon guidance have evolved in distantly related insects.
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影响因子：
2.7
作者：
Goltsev, Yury;Rezende, Gustavo L.;Vranizan, Karen;Lanzaro, Greg;Valle, Denise;Levine, Michael
通讯作者：
Levine, Michael
DOI：
10.1590/s0074-02762009000100020
发表时间：
2009-02-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Farnesi, Luana Cristina;Martins, Ademir Jesus;Rezende, Gustavo Lazzaro
通讯作者：
Rezende, Gustavo Lazzaro
DOI：
10.1073/pnas.0701515104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Adelman, Zach N.;Jasinskiene, Nijole;James, Anthony A.
通讯作者：
James, Anthony A.
影响因子：
16.2
作者：
Mitchell, KJ;Doyle, JL;Dickson, BJ
通讯作者：
Dickson, BJ
影响因子：
4.6
作者：
Goltsev, Yury;Fuse, Naoyuki;Levine, Mike
通讯作者：
Levine, Mike