A Myb transcription factor of Phytophthora sojae, regulated by MAP kinase PsSAK1, is required for zoospore development.
A Myb transcription factor of Phytophthora sojae, regulated by MAP kinase PsSAK1, is required for zoospore development.
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大豆疫霉的 Myb 转录因子受 MAP 激酶 PsSAK1 调节，是游动孢子发育所必需的
DOI：
10.1371/journal.pone.0040246
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Zhang M;Lu J;Tao K;Ye W;Li A;Liu X;Kong L;Dong S;Zheng X;Wang Y
PsSAK1是大豆疫霉（Phytophthora sojae）的一种丝裂原活化蛋白（MAP）激酶，在宿主感染和游动孢子活力中起重要作用。然而，PsSAK1的下游机制尚不清楚。本研究采用3′标记数字基因表达（DGE）分析方法对pssak1沉默突变体在包囊期和接种于易感大豆叶片组织后1.5 h的全局转录序列进行了测序。与受体大豆大豆菌株的基因表达水平相比，Myb家族的几个候选基因在PsSAK1缺失的情况下表达差异（上调或下调），包括r2r3型Myb转录因子PsMYB1。qRT-PCR结果显示PsSAK1沉默导致PsMYB1转录水平下降。PsMYB1的转录水平在产孢菌丝、萌发包囊和早期感染期间升高。PsMYB1的沉默导致三种表型：a)细胞质不分裂成单核游动孢子或释放正常游动孢子，b)孢子囊直接萌发，c)在游动孢子介导的植物感染中起作用。我们的数据表明，PsMYB1转录因子在MAP激酶PsSAK1的下游起作用，是豆豆虫孢子发育所必需的。
PsSAK1, a mitogen-activated protein (MAP) kinase from Phytophthora sojae, plays an important role in host infection and zoospore viability. However, the downstream mechanism of PsSAK1 remains unclear. In this study, the 3'-tag digital gene expression (DGE) profiling method was applied to sequence the global transcriptional sequence of PsSAK1-silenced mutants during the cysts stage and 1.5 h after inoculation onto susceptible soybean leaf tissues. Compared with the gene expression levels of the recipient P. sojae strain, several candidates of Myb family were differentially expressed (up or down) in response to the loss of PsSAK1, including of a R2R3-type Myb transcription factor, PsMYB1. qRT-PCR indicated that the transcriptional level of PsMYB1 decreased due to PsSAK1 silencing. The transcriptional level of PsMYB1 increased during sporulating hyphae, in germinated cysts, and early infection. Silencing of PsMYB1 results in three phenotypes: a) no cleavage of the cytoplasm into uninucleate zoospores or release of normal zoospores, b) direct germination of sporangia, and c) afunction in zoospore-mediated plant infection. Our data indicate that the PsMYB1 transcription factor functions downstream of MAP kinase PsSAK1 and is required for zoospore development of P. sojae.
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作者：
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PsSAK1 是大豆疫霉 (Phytophthora sojae) 的一种胁迫激活 MAP 激酶，是游动孢子活力和大豆感染所必需的
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通讯作者：
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影响因子：
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作者：
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