Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.
Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.
复制标题
增强微波浪图案表面上的细胞粘附和排列。
DOI：
10.1371/journal.pone.0104502
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Hu J;Hardy C;Chen CM;Yang S;Voloshin AS;Liu Y
已经制造出各种微图案并用于调节细胞黏附、形态和功能。通过标准光刻工艺制造的微图案通常是带有尖角的矩形通道（微沟槽），其对细胞的控制有限，不利于细胞间的相互作用和通讯。本文提出一种具有平滑波浪表面（微波浪）的新型微图案来控制细胞的位置和方向。为了表征细胞在微图案化基底上的生长和反应，将牛主动脉内皮细胞接种在带有微沟槽和微波浪的表面上培养24小时。结果表明，与微沟槽图案上的细胞相比，微波浪图案上的细胞死亡率更低且排列更好。此外，施加流动诱导的剪切应力来检测细胞在微波浪图案上的黏附强度。结果显示，与在平面上的细胞相比，黏附在波浪表面的细胞排列和黏附强度均得到改善。微波浪图案上细胞排列的改善、死亡率的降低以及黏附强度的增强相结合，将在细胞表型、增殖和组织工程的潜在应用中具有优势。
Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.
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影响因子：
14
作者：
Hsiao, Amy Y.;Torisawa, Yu-suke;Tung, Yi-Chung;Sud, Sudha;Taichman, Russell S.;Pienta, Kenneth J.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
影响因子：
4.1
作者：
Hakkinen, Kirsi M.;Harunaga, Jill S.;Yamada, Kenneth M.
通讯作者：
Yamada, Kenneth M.
影响因子：
14
作者：
Lam, Mai T.;Clem, William C.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
影响因子：
3.2
作者：
Choudhury, Deepak;Mo, Xuejun;Yu, Hanry
通讯作者：
Yu, Hanry
DOI：
10.1088/0960-1317/18/6/067001
发表时间：
2008-06-01
影响因子：
2.3
作者：
Eddings, Mark A.;Johnson, Michael A.;Gale, Bruce K.
通讯作者：
Gale, Bruce K.